Application of gas chromatography/negative ion chemical ionization mass spectrome try to legal medicine

负离子化学电离(NICI)质谱仪(MS)的一些优点已被报道，但这些优点取决于要分析的化合物的性质。本研究对苯二氮卓类药物及其酸解产物二苯甲酮和海因等神经精神药物的NICI、正离子电子碰撞(PIEI)和正离子化学电离(PICI)模式下的质谱图进行了记录和分析。对24种二苯甲酮类药物，NICI模式的灵敏度并不比PIEI和PICI模式好，但对药物结构中的卤素基团的识别非常有用。14个二苯甲酮类化合物的NICI模式的质谱图显示出卤素和硝基的阴离子，并且有非常强的分子阴离子对其进行灵敏的检测。海因类化合物的质谱图在m/z42，可用于药物群的筛选。除了上述质谱分析外，还对一些药物和毒物的各种提取方法进行了测试，如苯二氮卓类药物、小米妥酸盐、丁苯酮和有机磷杀虫剂。结果表明，Sep-Pak C18色谱柱具有提取速度快、回收率高、纯度高等优点，提取效果最好。上述数据在一些期刊上已经出现或是新出现的

Some advantages in the use of negative ion chemical ionization (NICI) mass spctrometry (MS)have been reported,but they depend on the nature of compounds to be analyzed. In this study,mass spectra in the NICI, positive ion electron impact(PIEI)and positive ion chemical ionization(PICI)modes were recorded and analyzed for neuropsychopharmaceuticals such as benzodiazepines,their acid-hydrolysis product benzophenones and hydantoins.The NICI mode was found not superior in sensitivity than PIEI and PICI modes for 24benzophenones,but is was very useful for identification of halogen groups in drug structures.The mass spectra of 14 benzophenones in the NICI mode showed anions due to halogen and nitro groups, and also very intense molecular anions useful for their sensitive detection by selected ion monitoring.The mass spectra of hydantoins showed a characteristic anions at m/z42, which can be used for screening of the drug group . In addition to the above mass spectral analyses various extraction methods were tested for a number of drugs and poisons such as benzodiazepines, berbiturates,butyrophenones and organophosphate pecticides. As a result, extraction with use of Sep-Pak C18 cartridges was best in view of its rapidness,high recovery and high purity. The above data have appeared or are new in press in some journals