Investigation of the pathgenesis of SSPE by use of murine monoclonal antibodies against measles virus polypeptides.

采用杂杂瘤技术获得了抗麻疹病毒多肽HA69K、NP60K、F40K、m36k的小鼠单克隆抗体，这些单克隆抗体不仅能裂解感染麻疹病毒多肽，而且能与细胞内麻疹病毒多肽发生特异性反应。应用免疫荧光技术对2例麻疹巨细胞肺炎死亡患者的肺组织进行特异性染色。对2例sspe患者（女孩14岁，男孩8岁）的脑脊液进行电泳、免疫学和病毒学分析。在这些脑脊液样品中检测到高滴度的麻疹特异性抗体和寡克隆IgG条带，但用小鼠单克隆抗体免疫荧光染色未发现这些脑脊液淋巴细胞中有病毒特异性多肽。

Murine monoclonal antibodies against measles virus polypeptides - anti HA69K, anti NP60K, anti F40K, anti M 36K - were obtained hybridoma technique which were specifically reacted with intracellular measles virus polypeptides not only by lytic infecton but also by persistent infection.Using these antibodies, lung tissues of two patients died with measles giant cell pneumonia were specifically stained by immunofluorescent technique.CSF samples of two patients with SSPE-14 year girl and 8 year boy-were obtained and were analyzed by electrophoretical, immunological and virological methods. High titer measles specific antibodies and oligoclonal IgG bands were detected in these CSF samples but, no virus specific polypeptides in lymphocytes of these CSFs were stained by immunofluorescence using murine monoclonal antibodies.