Lymphocyte Subsets and Tissue Destruction in Periodontitis

1.牙周炎患者淋巴细胞亚群分析。幼年型和急进型牙周炎患者外周血中T细胞比例低于正常对照组，B细胞比例高于正常对照组。牙周炎患者的血免疫球蛋白水平也高于正常人。这些偏离正常范围的数值随着临床症状的改善而恢复。青少年牙周炎患者的结果与成人牙周炎患者的结果不同。本研究结果提示，幼年或急进性牙周炎患者对牙周病原体的免疫应答可能与成人牙周炎患者或正常人不同。牙周炎的组织破坏：破骨细胞性骨吸收的机制。(1)白细胞介素1β(IL-1β)…破骨细胞中有更多的受体。用重组IL-1β和抗IL-1单抗，用免疫荧光和免疫电镜法检测体外培养破骨细胞上IL-1β受体的存在。该单抗可识别加入破骨细胞并与IL-1β受体结合的IL-1β。IL-1β结合部位密集分布于微绒毛泡、褶皱。(2)破骨细胞骨架与骨吸收活性。F-肌动蛋白被认为是直的或轻微弯曲的细丝，有时平行于细胞的外围。大量含有F-肌动蛋白的点排列在靠近细胞外围的条带中。牙周炎的遗传调控(1)牙周炎患者PBL的双色流式细胞术分析成人牙周炎患者PBL的双色分析显示，成人牙周炎患者外周血中CD4^+Leu8^+细胞的百分率略高于对照组，而CD8^+Leu8^-、CD8^+CD11b^+和CD8^+CD11b^-细胞的百分率略低于对照组。(2)牙周炎患者的人类白细胞抗原分型31例牙周炎患者中有20例为HLADUL抗原携带者。31例患者中有9例有人类白细胞抗原B_(W51)，而32例正常人中仅有2例有该抗原。较少

1. Analysis of Lymphocyte Subsets in Periodontitis Patients. The proportion of T-cells in peripheral blood lymphocytes(PBL) in juvenile or rapidly progressive periodontitis patients was lower than that of normal subjects, and the proportion of B-cells was higher than the control. The value of blood immunoglobulin in the periodontitis patients was also higher than that of the normal subjects. These deviated values from normal range were recovered with the improvement of clinical symphtoms. The present results from juvenile periodontitis patients were different from the results obtained from adult periodontitis patients. The present results have suggested that the immunological responses to periodontal pathoges in patients with juvenile or rapidly progressive periodontitis may be different from those in patients with adult periodontitis or those in normal individuals.2. Tissue destruction in Periodontitis: The mechanism of osteoclastic Bone Resorption. (1) Interleukin-1 beta (IL-1 beta) … More Receptors in Osteoclasts. The presence of IL-1 beta receptors on isolated osteoclasts were examined using immunofluorescent and immunoelectromicroscopic techniques with recombinant IL-1 beta and anti-IL-1 monoclonal antibody. The monoclonal antibody recognized the IL-1 beta which was added to the osteoclasts and bound to IL-1 beta receptors. IL-1 beta binding sites were densely distributed especially at microvilli blebs, ruffles. (2) Osteoclast Cytoskeleton and Bone Resorptive Activity. F-actin was seen as straight or gently curving filaments sometiages running parallel to the Cell periphery. A large number of F-actin containing dots are arranged in a band close to the cell periphery.3. Genetic Regulation of Periodontitis (1) Tow Color Flow Cytometry Analysis of PBL in Periodontitis Patients Two color analysis of PBL from adult periodontitis patients revealed that the percentages of CD4^+Leu8^+ cells was slightly higher than the control, while the percentages of CD4^+Leu8^-, CD8^+CD11b^+ and CD8^+CDllb^- cells were slightly lower than the control. (2) HLA Typing of Periodontitis Patients Twenty out of 31 patients with periodontitis were HLA-DUL antigen carriers. Nine of 31 patients had HLA-B_w51 antigen, while only 2 of 32 normal individuals showed the antigen. Less