Study for the effects of neuroactive peptides newly isolated
Study for the effects of neuroactive peptides newly isolated
批准号:
04044075
负责人:
TAKEUCHI Hiroshi
金额:
$3.07万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for international Scientific Research
财政年份:
1992
资助国家:
日本
项目状态:
已结题
起止时间:
1992 至 1994
中文摘要
本研究采用电生理学方法,对生物活性肽对非洲大蜗牛(Achatina fulica Ferussac)可识别的巨神经元的影响进行了全面的研究。而对哺乳类神经递质肽类如P物质、甲硫氨酸脑啡肽、神经降压素等不敏感,除了催产素另一方面,这些神经元对从无脊椎动物中分离的神经活性肽敏感。这些结果与小分子神经递质(如多巴胺、5-羟色胺和GABA)的结果不同,认为一种肽类神经递质对动物神经元的作用程度比小分子神经递质小。我们与三得利生物有机研究所和圣托马斯大学合作从Achatina神经节中分离出achatin-I(Gly-D-Phe-Ala-Asp),并对Achatin-I的兴奋作用进行了研究。APGW-酰胺(Ala-Pr 关于我们 o-Gly-Trp-NH_2)也是我们从Achatina神经节中分离到的被抑制的Achatina巨神经元。这两种肽被认为是Achatina神经元的神经递质。此外,我们还发现另一组从Achatina神经节中分离的Achatina cardiac-excitatory peptide-1(Ser-Gly-Gln-Ser-Trp-Arg-Pro-Gln-Gly-Arg-Phe-NH_2)对Achatina巨神经元有兴奋作用,而另一组从Achatina神经节中分离的Fulicin(Phe-D-Asn-Gln-Phe-Val-NH_2)对这些神经元有抑制作用。我们还证明了FMRFFamide(Phe-Met-Arg-Phe-NH_2),AF1从蛔虫中分离的FMRFamide的相关化合物(Lys-Asn-Glu-Phe-Ile-Arg-phe-NH_2)和[Ser^2]-贻贝抑制肽([Ser^2]-MIP)(Gly-Ser-Pro-Met-Phe-Val-NH_2),对achatin-I兴奋作用的细胞内信号转导系统和fulicin抑制作用的细胞内信号转导系统进行了电生理学研究。H-89(蛋白激酶(PK)A抑制剂)和W-7(钙调素抑制剂)可抑制achatin-I的作用,IBMX(环AMP磷酸二酯酶抑制剂)可促进achatin-I的作用,表明achatin-I通过环AMP-PKA系统发挥作用,钙调素参与了该系统。而KT-5823(PKG抑制剂)、calphostin C(PKC抑制剂)和W-7则可抑制Fulicin的抑制作用,表明Fulicin通过环GMP-PKG系统和/或IP_3和DG-PKC系统发挥作用,钙调素参与了这些系统。IBMX可抑制这种抑制作用,其机制目前尚不清楚。我们证明APGW-酰胺和achatin-I对Achatina神经元不仅是一种神经递质,而且也是一种神经调质。少
英文摘要
This year, we studied comprehensively effects of biologically-active peptides on the identifiable giant neurones of an African giant snail (Achatina fulica Ferussac) by the electrophysiological methods. The Achatina giant neurones were insensitive to the mammalian neurotransmitter peptides, such as substance P,Met-enkephalin, neurotensin, etc., except for oxytocin. On the other hand, these neurones were sensitive to the neuroactive peptides isolated from invertebrates. These findings were different from those of small molecule putative neurotransmitters, such as dopamine, serotonin and GABA.With these results, it was considered that one peptide neurotransmitter acts on the neurones of the less extent of animal species than the small molecule putative neurotransmitter. Achatina giant neurones were excited by achatin-I (Gly-D-Phe-Ala-Asp), isolated from Achatina ganglia by the collaboration of us, Suntory Institute for Bioorganic Research and University of Santo Tomas. APGW-amide (Ala-Pr … More o-Gly-Trp-NH_2), isolated also from Achatina ganglia by us, were inhibited Achatina giant neurones. These two peptides were proposed to be neurotransmitters for Achatina neurones. Besides, we demonstrated that Achatina cardio-excitatory peptide-1 (ACEP-1) (Ser-Gly-Gln-Ser-Trp-Arg-Pro-Gln-Gly-Arg-Phe-NH_2) isolated from Achatina ganglia by another group showed excitatory effects on Achatina giant neurones, and that fulicin (Phe-D-Asn-Gln-Phe-Val-NH_2) isolated from Achatina ganglia by another group had inhibitory effects on these neurones. We demonstrated also that FMRFFamide (Phe-Met-Arg-Phe-NH_2) isolated from the clam ganglia, AF1 (Lys-Asn-Glu-Phe-Ile-Arg-phe-NH_2), a related compound of FMRFamide isolated from an Ascaris, and [Ser^2]-Mytilus inhibitory peptide ([Ser^2]-MIP) (Gly-Ser-Pro-Met-Phe-Val-NH_2) isolated from Mytilus, had inhibitory effects on these neurones.The intracellular signal transduction systems for the excitatory effects of achatin-I and the inhibitory effects of fulicin were studied electrophysiologically using the inhibitors for these systems. H-89 (protein kinase (PK) A inhibitor) and W-7 (calmodulin inhibitor) inhibited the achatin-I effects, and IBMX (cyclic AMP phosphodiesterase inhibitor) facilitated the same effects, indicating that achatin-I acted via cyclic AMP-PKA system, and calmodulin participated in this system. On the other hand, KT-5823 (PKG inhibitor), calphostin C (PKC inhibitor) and W-7 suppressed the inhibitory effects of fulicin, indicating that fulicin acted via cyclic GMP-PKG system and/or IP_3 and DG-PKC systems, and calmodulin participated in these systems. IBMX suppressed the inhibitory effects, the mechanism of which cannot be explained at present.We demonstrated that APGW-amide, as well as achatin-I,acted not only as a neurotransmitter but also as a neuromodulator for Achatina neurones. Less
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TAKEUCHI,H.,Kim,K.H.,Liu,G.J.,YASUDAKAMATANI,Y.,Mi: "Achatin-I,an excitatory neurotransmitter having a D-phenylalanine residue of Achatina giant neurones" In″Invertebrate Neurobiology″,Akademiai Kiudo,Budapest,
TAKEUCHI,H.,Kim,K.H.,Liu,G.J.,YASUDAKAMATANI,Y.,Mi:“Achatin-I,一种兴奋性神经递质,具有 Achatina 巨型神经元的 D-苯丙氨酸残基”,《无脊椎动物神经生物学》,Akademiai Kiudo,布达佩斯,
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Santos,D.E.,Takeuchi,H.: "Influence of the drugs for membrane excitability modification on the excitation caused by achatin-I" Comparative Biochemistry and Physiology.
Santos,D.E.,Takeuchi,H.:“膜兴奋性修饰药物对 achatin-I 引起的兴奋的影响”比较生物化学和生理学。
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Santos D.E.and Takeuchi H.: "Influence of drugs for membrane excitability modification on the excitation caused by achatin-I." Comparative Biochemistry and Physiology. 105C. 185-188 (1993)
Santos D.E. 和 Takeuchi H.:“膜兴奋性修饰药物对 achatin-I 引起的兴奋的影响。”
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Liu,G.J.,Takeuchi,H.: "Effects of cyclic AMP,cyclic GMP and IP_3 intracellularly injected into the identifiable Achatina giant neu.." Comparative Biochemistry and Physiology.
Liu,G.J.,Takeuchi,H.:“细胞内注射环 AMP、环 GMP 和 IP_3 到可识别的 Achatina 巨神经元中的效果..”比较生物化学和生理学。
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Takeuchi H., Araki Y., Emaduddin M., Zhang W., Han X.Y., Salunga T.L.and Wong S.M.: "Identifiable giant neurones : their localizations in ganglia, axonal pathways and pharmacological features." Gen.Pharmacol.(in press).
Takeuchi H.、Araki Y.、Emaduddin M.、Zhang W.、Han X.Y.、Salunga T.L. 和 Wong S.M.:“可识别的巨型神经元:它们在神经节、轴突通路和药理学特征中的定位。”
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共 13 条
Novel function of the signaling molecule, PRIP.
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批准号:21791807
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项目类别:Grant-in-Aid for Young Scientists (B)
-
资助金额:$2.83万
-
财政年份:2009
-
负责人:TAKEUCHI Hiroshi
-
依托单位:
Study on Development of Support System for Healthcare Against Metabolic-Syndrome
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批准号:20300222
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$12.23万
-
财政年份:2008
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负责人:TAKEUCHI Hiroshi
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依托单位:
Roles of a novel signaling molecule in salivary secretion
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批准号:19791368
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项目类别:Grant-in-Aid for Young Scientists (B)
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资助金额:$2.36万
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财政年份:2007
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负责人:TAKEUCHI Hiroshi
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依托单位:
Improvement of a geometry optimization method for molecular clusters and its application
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批准号:19550001
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.0万
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财政年份:2007
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负责人:TAKEUCHI Hiroshi
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依托单位:
Personal Dynamic Healthcare System Utilizing Mobile Phone and Web technologies
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批准号:16500457
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.37万
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财政年份:2004
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负责人:TAKEUCHI Hiroshi
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依托单位:
studies on the relationship of the structure of manifolds and p-harmonic functions
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批准号:16540208
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.2万
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财政年份:2004
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负责人:TAKEUCHI Hiroshi
-
依托单位:
The properties of P-harmonic maps and the application to Geometry
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批准号:11640221
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.11万
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财政年份:1999
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负责人:TAKEUCHI Hiroshi
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依托单位:
Pollution of molluscicide and concentration in organs.
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批准号:07045047
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项目类别:Grant-in-Aid for international Scientific Research
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资助金额:$0.96万
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财政年份:1995
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负责人:TAKEUCHI Hiroshi
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依托单位:
Signal transduction systems of achatin-I,a neuropeptide having a D-phenylalanine residue.
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批准号:06680758
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项目类别:Grant-in-Aid for General Scientific Research (C)
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资助金额:$0.96万
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财政年份:1994
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负责人:TAKEUCHI Hiroshi
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依托单位:
Neurophysiological and neuropharmacological investigations of achatin-I, a neuroactive peptide having a D-Phe residue.
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批准号:02670049
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项目类别:Grant-in-Aid for General Scientific Research (C)
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资助金额:$1.41万
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财政年份:1990
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负责人:TAKEUCHI Hiroshi
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依托单位:
Study for the isolation of neuroactive peptides from the ganglia of Achatina fulica Ferussac.
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批准号:02044061
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项目类别:Grant-in-Aid for international Scientific Research
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资助金额:$2.88万
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财政年份:1990
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负责人:TAKEUCHI Hiroshi
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依托单位:
Separation of Proteins by a Liquid Membrane with Reverse Micelles
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批准号:01550730
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项目类别:Grant-in-Aid for General Scientific Research (C)
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资助金额:$1.34万
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财政年份:1989
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负责人:TAKEUCHI Hiroshi
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依托单位:
One-step Amination by Parent Nitrenium Ion and Alkylnitrenium Ion
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批准号:63490013
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项目类别:Grant-in-Aid for General Scientific Research (B)
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资助金额:$4.22万
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财政年份:1988
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负责人:TAKEUCHI Hiroshi
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依托单位:
Active Transport of Heavy Metal Ions through Polymeric Blended Membranes
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批准号:62550694
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项目类别:Grant-in-Aid for General Scientific Research (C)
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资助金额:$1.28万
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财政年份:1987
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负责人:TAKEUCHI Hiroshi
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依托单位:
Lymphocyte Subsets and Tissue Destruction in Periodontitis
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批准号:62570820
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项目类别:Grant-in-Aid for General Scientific Research (C)
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资助金额:$1.47万
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财政年份:1987
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负责人:TAKEUCHI Hiroshi
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依托单位:
Mechanism of Degradation of Liquid Membranes and Washout Characteristics of the Membrane Liquids
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批准号:60550663
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项目类别:Grant-in-Aid for General Scientific Research (C)
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资助金额:$0.45万
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财政年份:1985
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负责人:TAKEUCHI Hiroshi
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依托单位:
Reactivities of Nitrenium Ion Intermediates and the Application to Organic Syntheses
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批准号:60490007
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项目类别:Grant-in-Aid for General Scientific Research (B)
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资助金额:$3.14万
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财政年份:1985
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负责人:TAKEUCHI Hiroshi
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依托单位:
海外基金