Studies on canine erythrocyte insulin receptors

为探讨犬糖尿病、肾上腺皮质功能亢进等疾病中细胞胰岛素不敏感的机制，对红细胞胰岛素受体进行了检测。确定了犬红细胞胰岛素受体测定的最佳条件：3种孵育温度(4ﾟC、15ﾟC、37ﾟC)和不同的孵育时间(0~8h)。实验结果表明，犬红细胞胰岛素受体测定的最佳条件为孵育温度为4ﾟ℃，孵育时间为6h。在此条件下，犬红细胞胰岛素受体空位(Ke)和填充位(Kf)的胰岛素受体数目及其亲和力分别为0.297±0.117/ng和0.045±0.008/ng。自发性糖尿病：5例自发性糖尿病犬较临床健康犬升高(159.0±167.3，63.4~456.6)，KE(0.017±0.010/ng)和KF(0.009±0.005/ng)降低。有3例患者胰岛素治疗后血细胞减少(治疗前分别为110.2、89.6、75.4，治疗后分别为21.2、11.0、49.8)，KE、KF水平升高。在肾上腺皮质功能亢进的情况下，3例地塞米松诱导的肾上腺皮质功能亢进患者胰岛素受体数量减少，亲和力(Ke和Kf)增加，同时观察到葡萄糖消失率和/或高胰岛素血症。8例自发性肾上腺皮质功能亢进症患者的红细胞胰岛素受体数目、Ke和Kf水平均无明显差异。

For investigation on the mechanism of the cellular insulin insensitivity in some disorders, such as diabetes mellitus and hyperadrenocorticism in dogs, the erythrocyte insulin receptors were examined as follows.1. The optimal condition for the measurement of canine erythrocyte insulin receptors: 3 incubation temperatures (4ﾟC, 15ﾟC, and 37ﾟC) and various incubation periods (from 0 to 8 hours) were examined. The results of this experiment revealed that the optimal condition for the measurement of canine erythrocyte insulin receptors was at 4ﾟC as incubation temperature and for 6 hours as incubation period. Under these conditions, the number of insulin receptors and their affinity in empty site(Ke) and the filled site(Kf) of insulin receptors were 21.22+10.9/cell, and 0.297+0.117/ng and 0.045+0.008/ng in canine erythrocyte, respectively.2. In the case of spontaneous diabetes mellitus: in 5 cases of spontaneous diabetes-dogs, the number(159.0+167.3, range 63.4-456.6) was higher, and the Ke(0.017+0.010/ng) and Kf(0.009+0.005/ng) levels were lower than those of clinically healthy dogs. After insulin treatment on 3 cases, the number was decreased(before;110.2, 89.6, and 75.4, after;21.2, 11.0, and 49.8, respectively), and Ke and Kf levels were increased.3. In the case of hyperadrenocorticism; in 3 cases of dexamethasone induced hyperadrenocorticism, the number of insulin receptors was decreased and the affinity(Ke and Kf) was increased, while the glucose disappearance rate and/or hyperinsulinism were observed. In 8 cases of spontaneous hyperadrenocorticism, no difference in the number, Ke, and Kf levels of erythrocyte insulin receptors were observed.