How is intracellular calcium ion in parathyroidal tumor cells increased?

在刺激-分泌耦合中，刺激诱导细胞内钙离子的增加，随后通过逆胞饮机制分泌分泌颗粒。没有激素控制甲状旁腺主细胞，然后通过细胞外钙离子的增加或减少来决定甲状旁腺激素（PTH）的分泌。在本研究中，测量。探讨了细胞内钙离子转移和钙离子转移与甲状旁腺激素分泌的关系。将人及小牛甲状旁腺瘤组织切碎，用0.05%胶原酶处理，制备游离细胞。分散的甲状旁腺细胞在含有10%胎牛血清的Pulbecco最低基本培养基中培养，并置于皮氏培养皿的玻璃罩上。5 muM Fura-2AM载药处理2小时。洗涤后，将装有培养细胞的盖板玻璃置于恒温箱中，用联链平衡盐溶液灌注。采用项目型荧光相衬显微镜，将一个细胞投影在340nm和380nm的发射点上。一个细胞的辐射通过510nm的带通滤波器，被超灵敏摄像机接收。摄像机上的细胞发射图像用示波器表示，并用笔录笔在纸上画出来。灌注液低钙刺激后5 msec内细胞内钙离子数量逐渐增加，并在前10 msec内迅速达到最高点。钙瞬时值在40 msec时逐渐降低至基底点。

In stimulation-secretion coupling, a stimulation induces the increase of intracellular calcium ions, subsequently following that the secretory granules are secreted by the mechanism of reverse pinocytosis. None hormone controls parathyroid chief cells and then by the increase or decrease of extracellular calcium ions the secretion of parathyroid hormone(PTH) is determined. In the present study, the measurement. of intracellular calcium ion transition and the relationship between calcium transition and secretion of PTH are investigated.The human and calf parathyroid tumor tissues were minced, and treated with 0.05% collagenase, subsequently resulting in preparing free cells. The dispersed parathyroid cells were cultured in the Pulbecco's minimum essential medium containing 10% fetal calf serum on a cover glass in Petri dish. They were treated with the loading of 5 muM Fura-2AM for 2 hours. After washing, the cover glass with the cultured cell was seton constant-temperature chamber, and perfused by lianks balanced salt solution. By project-type fluorescence phase-contrast microscopy, one cell was projected on emission of 340nm and 380 nm. The emission irradiated by one cell runs through 510nm band pass filter, and was received in ultra- sensitive video-camera. The images of the cell emission on the video-camera was expressed on oscillograph, and was drew on paper by penrecorder. The intracellular calcium ions was gradually increased in number for 5 msec after stimulation by hypocalcium concentration of perfusion fluid and them rapidly reached the highest point within the first 10 msec. The calcium transient was gradually decreased to basal point at 40 msec.