Possible effect of splenectomy on liver regeneration in cirrhotic rats.

目前的实验方案是确定脾切除对肝硬变大鼠肝再生的可能影响。首先，对肝切除过程中的病理生理变化进行了研究，重点研究了细胞动力学、膜流动性作为膜功能的变化。正常大鼠肝切除70%后6小时膜流动性增加，24小时达高峰。而用碘化丙啶和BrdU抗体进行双色分析的DNA直方图显示，肝切除后24小时和48小时出现S相核的高峰。为了产生虹膜，进行了为期10周的4%硫代乙酰胺的腹腔注射。随着肝硬变的发展，膜流动性下降，细胞动力学分析显示2C室比例较正常升高。通过对肝硬变大鼠进行70%的肝切除，大约三分之二的肝硬变大鼠在48小时内死亡。然而，脾切除并没有提高存活率。然后行50%肝切除，以提高存活率。通过。在正常大鼠肝部分切除后，膜流动性增加，有一半的大鼠出现S相核。然而，脾切除并未显著改变细胞膜流动性或细胞动力学。上述结果提示，即使在肝部分切除后，肝硬变也会发生再生过程，而脾切除对肝部分切除后肝细胞膜流动性或再生的影响不明显。

The present experimental protocol is to identify a possible effect of splenectomy on liver regeneration in cirrhotic rats. First, the pathophysiology along with hepatic resection was studied, paying an attention at changes in cell kinetics, membrane fluidity as membraneous function. In normal rats, membrane fluidity was increased at 6 hours after 70% hepatectomy, making a peak at 24 hours later. By contrast, DNA histogram using 2 color analysis with propidium iodide and BrdU antibody showed a peak of S-phased nuclei at 24 and 48 hours after hepatectomy. In order to produce eirrhosis, an intraperitoneal injection of 4% thioacetoamide was performed for 10 weeks. Along with development of cirrhosis, membrane fluidity decreased and the analysis of cell kinetics showed an increasing proportion of 2C compartment compared to that of normal. By 70% hepatectomy in the cirrhotic rats, about two of third cirrhotic rats died within 48 hours later. However, splenectomy didnot improve the survival rate. Then, 50% hepatectomy was undergone to improve survival rate. By. this, membrane fluidity was increased and also, S-phased nuclei appeared in half of proportion of normal rats after partial hepatectomy. However, splenectomy did not alter significantly membrane fluidity or cell kinetics. Above results suggest that regeneration process occurs even in cirrhotic liver after partial hepatectomy and splenectomy does not affect significantly the changes in membrane fluidity or regeneration following partial hepatectomy.