Kindling as a model of neuronal plasticity (Effect of dentate nucleus lesion upon amygdaloid kindling in rats)

淀粉样蛋白点燃用于研究小脑齿状核是否参与大脑的神经元可塑性。在本报告中，我们研究了在对照条件下和对侧或同侧小脑齿状损伤后，杏仁核核点燃过程中的行为变化、癫痫样事件的发生和放电后的持续时间。结果表明，对侧齿状损伤可促进杏仁核的行为发育，而杏仁核的自发性癫痫样电位仅略有增加。第二个发现是，在受到左齿状病变影响的右侧杏仁核上，有很少的尖峰，而在左侧杏仁核上，有频繁的尖峰。同侧齿状损伤组与对照组相比，杏仁核的点火发育和尖峰数量没有差异。但两组的后放电时间存在差异。在同侧和对侧点火的比较中，点火的发展和自发尖峰有显著差异，但后放电的持续时间没有差异。这些结果表明，齿状核在杏仁核的点燃以及杏仁核的神经元可塑性中起着重要作用。因此，齿状核在癫痫的泛化机制中起一定的控制作用。然而，任何一侧齿状损伤后放电的缩短可能引起齿状丘脑兴奋系统的减弱。如果在杏仁核点燃完成后进行齿状损伤，则点燃的发展、后放电的持续时间和自发峰的数量没有明显变化。

Amyidaloid kindling is used to investigate whether the cerebeller dentate nuc eus participates in the neuronal plasticity of the cerebrum. In this report we studied the behavioral change, the occurrence of epileptiform events and duration of afterdischarge during amygdaloid kindling under control conditions and after a dentate lesion of the contralateral or ipsilateral cerebellum. Our results indicate that the contralateral dentate lesion induced the facilitation of the behavioral development of the amygdaloid kindling, whereas, there was only a slight increase of the spontaneous epileptiform potential of the amygdala. A second finding was that on a right amygdala which had been influenced by a left dentate lesion, there were few spikes, while on the left amygdala there were frequent spikes. In the ipsilateral dentate lesioned group there was no difference of the development of kindling and number of spikes of the amygdala comparing to the control kindling. However there was a difference of the duration of afterdischarge. As to the comparison of the ipsilateral and contralateral kindling there were significant differences of the development of kindling and spontaneous spikes but no difference of the duration of afterdischarge. These results suggest that the dentate nucleus plays an important role in amygdaloid kindling as well as in the neuronal plasticity of the amygdala, specifically. Therefore the dentate nucleus must play a role in controlling the generalization mechanism of epilepsy. However, the shortening of the afterdischarge of the dentate lesion of either side may be caused the diminution of dentate-thalamic excitatory system.If the dentate lesion was done after the accomplishment of the amygdaloid kindling, there was no significant change of the development of kindling, the duration of afterdischarge and the number of spontaneous spikes.