Mechanisms of gestagen therapy on endometrial cancer.

本文试图阐明孕激素治疗子宫内膜癌和乳腺癌的机制。通过口服7，12-二甲基苯并[a]蒽（DMBA）在雌性Sprague-Dawley大鼠中诱导。组织学分高分化腺癌、中分化腺癌等7种类型，49例乳腺癌标本经葡聚糖-活性炭（DCC）法检测，雌激素受体（ER）阳性43例（87.8%），孕激素受体（PR）阳性34例（69.4%）。将DMBA诱导的乳腺肿瘤部分移植到裸鼠皮下，共38例。除了一个既不含ER也不含PR的样品外，所有样品都不能产生肿瘤。移植于大鼠皮下的肿瘤不生长，原代培养35次。虽然在原代阶段观察到上皮细胞和成纤维细胞的良好细胞生长，但尚未建立细胞系，因为细胞数量在每次传代培养时逐渐减少。本文研究了醋酸甲羟孕酮（MPA）对裸鼠移植瘤的影响。未观察到明显的抗肿瘤作用。我们还研究了MPA在体外使用该细胞株的抗肿瘤作用。MPA组与对照组之间无差异。

An attempt was made to clarify the mechanisms of gestagen therapy on endometrial cancer or breast cancer.Mammary tumor was. induced in female Sprague-Dawley rats by oral administration of 7, 12-dimethybenz[a]anthracene(DMBA). Histologically, the induced mammary tumors were divided into 7 types such as well differentiated adenocarcinoma, moderately differentiated adenocarcinoma and so on. Estrogen receptor(ER)and progesterone receptor(PR)were positively determined in 43(87.8%)and 34(69.4%), respectively, out of 49 samples by the dextran-coated charcoal(DCC)method. A portion of DMBA-induced mammary tumor was transplanted subcutaneously into nude mice in 38 samples. All but one sample which contains neither ER nor PR, could not make tumors. The tumors which were autoimplanted into subcutaneous tissue of the rats did not develop.The primary culture of the tumor have been carried out in 35 times. Though the excellent cell growth of the epithelial cells and fibroblastic cells were seen at the primary stage, no cell lines have been established, because the cell numbers had been tapering at each subculture. We investigated the effects of medroxyprogesterone acetate(MPA)on the tumor developed in nude mice. No apparent antitumor effects were seen. We also investigated antitumor effects of MPA in vitro using the cell strain. There were no difference between the MPA group and the control group.