Development of Masspropagation Systems for Tissue Culture

包括体细胞胚发生在内的组培苗批量生产的自动化是亟待解决的问题之一。在体胚生产中，建立了一套适合大小愈伤组织筛选的自动化系统，并进行了试验。对网片进行反洗可有效防止网片堵塞。虽然系统性能没有达到人工筛分的水平，但实验表明，本研究提出的基本思路对未来的系统是有效的。在体胚发生研究中，应用人工视觉系统是一种很有前途的方法。液体培养是一种非常流行的方法，它需要驯化以防止主要由于潮湿条件而导致的玻璃化。研究了营养雾培养为马铃薯植株生长带来的干燥环境。每10 min循环1 min的养分供给使植株鲜重和干重增加最多，根系发育最好。尽管观测到一些数据的散射，但预计进一步优化雾供应变化可能会带来更有效的传播。在4种不同相对湿度条件下培养番茄幼苗25 d，研究其蒸腾和净光合速率。单位叶面积净光合速率随相对湿度的增加而增加。对大蕙兰在光自养和光异养条件下的繁殖和生长进行了比较。光致变色条件下PLB的总干重较大。利用神经网络对温室水培番茄植株的水分和养分供应进行了识别。利用8小时的输入数据和4个单元组成的单个隐层，很好地计算了供水量和养分。

Automatization of the massproduction of tissue culture seedlings including somatic embryogenesis is one of the problems to be solved urgently. A prototype system was constructed and tested for automation of selecting procedure of appropriately sized callus by sieving in somatic embryo production. Back washing the mesh was found to be effective for preventing the mesh blockage. Although the system performance did not reach that of the manual sieving, the experiment suggested that the basic ideas developed in this study would be effective for the future system. The application of artificial vision system which demands for the detailed knowledge of morphology of somatic embryo is promising methodology in somatic embryogenesis.Acclimatization to prevent glassification mainly due to wet condition is needed for liquid culture which is very popular method. Nutrient mist culture which brings dryer environment was investigated for potato plantlet growth. Nutrient supply for 1 min. per a 10 min. cycle bought about the largest increase of fresh and dry weights and the best root development. It is expected that further optimization of the mist supply variations may bring about more efficient propagation, although some scattering of data was observed.Tomato plantlets were cultured for 25 days in four different relative humidity conditions to investigate the transpiration and net photosynthetic rates. Net photosynthetic rate per unit leaf area increased with increasing relative humidity. An experiment was conducted to compare the multiplication and growth of the Cymbidium PLB under photoautotrophic and photomixotrophic conditions. Total dry weight of the PLB was greater in photomixotropic condition.Water and nutrient supply to hydroponic tomato plants in a greenhouse id identified by using neural nets. By using 8 hour input data and single hidden layer which consists of 4units, the amount of water and nutrient supply was calculated well.