Practical application of micropartiulate diet in fish seed production

1. 结果表明，鱿鱼粉和褐鱼粉是河豚幼鱼微颗粒饲料中有效的蛋白质来源。2 .酪蛋白饲粮中添加丙酰脯氨酸、丙酰丝氨酸、丙酰甘氨酸、丙酰苯丙氨酸、丙酰脯氨酸甘氨酸等寡肽对红鲷幼鱼的生长有显著的促进作用。饲养试验表明，添加n-3高不饱和脂肪酸（HUFA）的饲料能提高阿育鱼幼鱼的增重。HUFA的作用大小依次为EPA + DHA<DHA<EPA。研究表明，饲料中的某些磷脂对于维持斑jack幼虫的生长和存活是不可缺少的。在添加2%大豆卵磷脂的饲粮中达到最高水平。不添加维生素C组比目鱼生长迟缓，45 d内死亡率高，而添加维生素C组比目鱼90 d后体重增长约50g。在50吨池中，用微颗粒饲料饲养6万只阿育鱼幼虫。用含有卵磷脂的微颗粒饲料饲养阿育鱼幼虫时，几乎没有出现脊柱侧凸等异常现象。将微颗粒饲料与活饲料相结合用于蓝蟹制种，其幼蟹成活率可达70%。用微颗粒饲料对牙鲆幼体进行了制种试验。10日龄成活率为58%，30日龄成活率为37%，60日龄成活率为32%。此外，使用微颗粒饲料可降低malfo=的发生率。

1. It was demonstrated that squid meal and brown fish meal ar. e an effective protein source of microparticulate diet for puffer fish larvae.2. The supplementation of casein diet with oligopeptides such as alanylproline, alanyl-serine, alanyl-glycil-glycine, alanyl-phenylalanine, alanyl-prolyl-glycine gave growth higher than only casein for red sea bream larvae.3. It was shown by feeding experiments that Ayu fish larvae gave a higher weight gain with diets containing n-3 highly unsaturated fatty acid (HUFA). The effect of HUFA was in order of EPA + DHA<DHA<EPA.4. Some phospholipids in diets were demonstrated to be indispensable for sustaining growth and survival of striped jack larvae. The highest was obtained on diets with 2% soybean lecithin.5. Japanese flounder receiving the diet without supplemental vitamin C retarded growth and resulted in high mortality within 45 days, whereas that receiving the diet containing vitamin C grew up about 50g in body weights after 90 days.6. Ayu larvae of 60, 000 were produced with microparticulate diet in 50-ton tank. Also, such abnormalities as scoliosis were rearely found when the Ayu larvae were reared with microparticulate diet containing lecithin.7. When the combination of microparticulate diet and live food was used in seed production of blue crab, larval crab reached juvenile crab in the survival rate of 70%.8. The seed production was conducted on the larval Japanese flounder with microparticulate diet. Fish larvae were produced in survival rate of 58% at 10-day old, 37% at 30-day old and 32% at 60-day old. Also, the incidence of malfo=ation was reduced by using microparticulate diet.