Studies on bilirubin metabolism in fish

1.对黄尾鱼部分组织的TBA值、α-Toc含量进行了分析。用高效液相色谱法和EIA法测定了黄尾鱼血浆中胆红素的含量。在活体中，黄斑鱼组织中的脂质过氧化产物进行性增加，因此称为蓝红素X在血浆中增加。这些结果证实，活性氧物种可能是黄疸的主要原因。用脂肪过氧化水平不同的3种褐鱼粉(I-111组)和9%POV为4.7(IV组)或100%(V组)的褐鱼粉饲喂黄尾鱼30天，分析肝脏中α-生育酚(α-Toc)含量、2-硫代巴比妥酸反应物质(TBARS)值及超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性的变化。体内脂质过氧化水平最高的饲料或含有过氧化脂质的饲料可使黄鱼肝脏发生脂质过氧化反应，抑制体内脂质过氧化反应可预防黄鱼黄疸的发生。1989年4月和6月，对养殖场养殖的团头鱼进行了高胆红素血症的观察。其原因可能与生殖有关。分析了黄尾鱼体内脂质过氧化和清除自由基能力的季节变化。这些结果解释了黄尾鱼的黄疸主要发生在夏季的原因。制备并鉴定了针对未结合胆红素-LXα的特异性抗胆红素-LXα的单抗。抗体只与胆红素-IXα反应，而不与结合的胆红素-LXα反应。用重氮法和抗胆红素-Lxα单抗EIA法测定了几种疾病的血清胆红素含量。对胆红素X的结构也进行了研究。结果表明，胆红素是最原始、最有效的自由基清除剂。

1. TBA values, content of alpha-Toc in some tissues of jaundiced yellowtail were analyzed. Bilirubin contents in the plasma of jaundiced yellowtail were analyzed by HPLC and EIA method. In vivh lipid peroxidadon progressed in the tissues of jaundiced fish and so call blirubin X increased in the plasma. These results confirm that the active oxygen species may be a piincipale cause of the jaundice.2. Yellowtail were fed on three brown fish meal diets whose lipid peroxide levels were deffemt (group I-111) or brown fishmeal 9 % of sardine oil whose POV was 4.7 (group IV) or 100 (group V) for 30 days and changes in a-tocopherol (alpha-Toc) contents, 2-thiobarbituric acid reactive substances (TBARS) values and superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities in the liver were analyzed. In vivo lipid peroxidaion may progress in the liver of yellowtail fed on diets whose lipid peroxidatin was the highest levels or which contain lipid peroxides and that the suppression of in vivo lipid peroxidation may prevent the jaundice of yellowtail.3. Highperbilirubinemia were observed in red sea bream obtained from fish farm in April and June, 1989. The cause may probably related to the reproduction.4. Seasonal changes of in vivo lipid peroxidation and free radical scavenging abilty of yellowtail were analyzed. Results obtained account for the reason that the jaundice of yellowtail occurred mainly in summer.5. Anti-bilirubin-lX alpha monoclonal antibodies exclusively specific for unconjugated bilirubin-lX alpha are prepared and characterized. The antibodies reacted only bilirubin-IX alpha but did not react with conjugated bilirubin-lX alpha.6. Bilirubin contents in the serum of several disease were analyzed by diazo method and EIA method in which antibilirubin-lX alpha monoclonal antibodies were used. Structure of so call bilirubin X were also studied. Results obtained indicate that bilirubin is the most primitive and effective free radical scavenger.