Descending projections of the hypothalamic paraventricular nucleus

研究了下丘脑室旁核（PVN）对A5肾上腺素能神经元、上涎核神经元和交感神经节前神经元的下行投射。1) pvn向上唾液腺核的投射：pvn纤维通过PHA-L的顺行转运进行标记，而上唾液腺核神经元则通过HRP逆行标记，并应用于岩大神经。儿茶酚胺合成酶（DBH）免疫组化检测A5肾上腺素能神经元。pvn -纤维只具有静脉曲张。在上唾液腺核区域内发现，而与A5肾上腺素能神经元密切相关的终末或静脉曲张偶见。2) pvn纤维到交感神经节前神经元：pvn纤维用PHA-L顺行标记，交感神经节前神经元用注射于颈上神经节的胆曲霉毒素B亚基逆行标记。pvn纤维的末端和静脉曲张偶见与位于中央椎管背侧的中央自主神经区交感神经节前神经元树突相邻。这些结果表明下丘脑室旁核参与调节自主神经系统的活动，包括副交感神经和交感神经。

The descending projections from the hypothalamic paraventricular nucleus(PVN)to the A5 adrenergic neurons, the superior salivatory nucleus neurons and the sympathetic preganglionic neurons were studied.1)PVN-projections to the superior salivatory nucleus : PVN-fibers were labeled by anterograde transport of PHA-L, while superior salivatory nucleus neurons were retrogradely labeled by HRP which was applied to the greater petrosal nerve. A5 adrenergic neurons were demonstrated by immunohistochemistry for catecholamine synthetizing enzyme(DBH). PVN-fibers with varicosities were exclusively. found within the area of the superior salivatory nucleus, while terminals or varicosities were occasionally observed in close relation to A5 adrenergic neurons.2)PVN-fibers to the sympathetic preganglionic neurons : PVN-fibers were labeled anterogradely by PHA-L, while the sympathetic preganglionic neurons were labeled retrogradely by choleratoxin subunit B which was injected in the superior cervical ganglion. PVN-fibers with terminals and varicosities were occasionally found in contiguity with the dendrites of the sympathetic preganglionic neurons which were located in the central autonomic area dorsal to the central canal.These results suggest that the hypothalamic paraventricular nucleus is involved in regulation of activities of the autonomic nervous system, both oi parasympathetic and sympathetic nerves.