Morphological investigations of glucose uptake in the mouse hippocampus.

近年来，人们对能量代谢与大脑神经活动之间的关系越来越感兴趣。我们一直在研究小鼠海马体中葡萄糖摄取的因素。我们将在此报告海马各层状区微血管、层状胺、胰岛素、胰岛素受体和葡萄糖转运体的密度。与其他区域相比，墨汁灌注微血管密度最高的是凹层-分子层，最低的是透明层和齿状颗粒细胞层。锥体细胞层中，CA3a扇区微血管密度显著高于CA1扇区。虽然免疫组织化学染色的层胺分布与微血管一致，但在锥体和颗粒细胞层也可见免疫阳性反应。相反，胰岛素和胰岛素受体集中分布在颗粒层和锥体层，而在空隙层-分子层中分布最少，这表明胰岛素不参与葡萄糖进入大脑的运输。葡萄糖转运蛋白的免疫组化反应不仅在微血管壁高度阳性，而且在空洞层-分子的实质组织中也高度阳性。重度缺氧（NO_2 30min）对大鼠海马星形胶质细胞的突触密度和形态变化无明显影响。

In recent years, there has been a growing interest in the relationships between energy metabolism and neural activity in the brain. We have been investigating the factors responsible for glucose uptake in the mouse hippocampus. We will report here the densities of microvessel, laminine, insulin, insulin receptor and glucose transporter in each laminar region of the hippocampus. The highest density of perfused microvessels with indian ink was found in the stratum lacunosum - moleculare, as compared with other regions, and lowest values were found in the stratum lucidum and dentate granular cell layer. Among pyramidal cell layers, microvessel density of the sector CA3a was significantly higher than that of the CA1. Although laminine distributions stained immunohistochemically were consistent with that of microvessels, immuno - positive reactions were also seen in the pyramidal and granular cell layer. By contraries, insulin and insulin receptors were intensively distributed in the stratum granulare and pyramidale, while they were lowest in the stratum lacunosum - moleculare, suggesting that the insulin have no dealings with the glucose transport into the brain. The immunohistochemical reactions of glucose transporter, responsible for facilitated glucose transport in the brain, were not only highly positive in the microvessel wall, but also in the parenchymal tissue in the stratum lacunosum - moleculare. The severe anemic hypoxia(NO_2 for30 minutes), used for the model of aging, did not any influence to the synaptic densities and morphological changes of astroglial cells in the hippocampus.