The Role of Fimbriae of Escherichia coli in Genitourinary Tract Infection.

Escherichia coli isolated from patients with acute uncomplicated infections的细菌性质主要被测试为澄清fimbriae的显著性作为E的病毒因子。大肠杆菌中的遗传感染。然后，MS和MR fimbriae包括P的相似存在被认为是genitourinary infections中最重要的病毒因子之一。作者开发了一个新的实验系统，使用一个alloxan induced糖尿病小鼠和一个非常小的体积in order to analyze the establishment of infection。三种变体是由OEC-795开发的，其中有Flagella （F）和MS Fimbriae。使用父母（MS+·F+）和三个变体（MS+·F-、MS-·F+、MS-·F-），MS fimbriae的角色在这个实验系统中重新测试。从某种意义上说，在感染的建立中，MS fimbriae的重要性是明确的。MS-fimbriated E受体的分布。人类超外层细胞上的大肠杆菌也被测试过。MS-fimbriated E.大肠杆菌被吸收到一个超金属细胞的表面，除了它的黑色发光超级细胞。天冬氨酸-连接糖蛋白的高锰寡链肽的分布，即MS-fimbriae的实际受体动力学，主要用反向散射模式的扫描电镜分析，使用胶体金聚合的Con A （G-Con A）。G-Con A的极罕见分布被观察到了Bladder Luminal Cell的表面。However，G-Con A的一个相对密集的分布被检测到了正常脱水机和再生性阴茎的表面，但不用于对超甲状腺癌的治疗。

Bacterial properties of Escherichia coli isolated from patients with acute uncomplicated infections were examined mainly to clarify the significance of fimbriae as the virulence factor of E. coli in genitourinary tract infections. Then, a simultaneous presence of MS and MR fimbriae including P is considered to be one of the most important virulence factors in genitourinary infections.The author developed a new experimental system using an alloxan induced diabetic mouse and an extremely small volume of inoculum in order to analyze the first event of the establishment of infection. Three variants were developed from OEC-795, which possessed flagella (F) and MS fimbriae. Using the parent (MS+・F+) and three variants (MS+・F-, MS-・F+, MS-・F-), the role of MS fimbriae was re-examined in this experimental system. Consequently, the significance of MS fimbriae in the establishment of infection was demonstrated clearly.The distribution of receptor for MS-fimbriated E. coli on human urothelial cells was also examined. MS-fimbriated E. coli adhered to the surface of urothelium except for that of the bladder luminal superficial cell. The distribution of high-mannose oligosaccharide of asparagine-linked glycoprotein, as an actual receptor moiety for MS-fimbriae was analyzed mainly with backscatter mode of SEM using colloidal gold conjugated Con A (G-Con A). Extremely rare distribution of G-Con A was observed on the surface of the bladder luminal cell. However, a relatively dense distribution of G-Con A was detected on the surfece of normal ureter and renal pelvis not to mention on that of urothelial cancer.