Membrane Potentials and Their Distribution Pattern of Inflammatory Cells Collected from Acute Periodontitis

大多数关于急性牙周炎炎症细胞的研究都与该问题的形态学或免疫学方面有关。虽然膜电位能反映细胞功能，但电生理学方法尚未引起研究者的重视。为了建立急性牙周炎细胞膜电位的测量方法，并将其与细胞功能联系起来，本研究采用腹腔渗出液和脾脏细胞测量细胞内电位，并对测量系统进行校准。用玻璃微毛细管从人牙龈沟液（GCF）中收集炎性细胞。然后将细胞移植到含有10%FCS的MEM肉汤中，并在5%二氧化碳气氛中孵育30至60分钟。将装有3 M KCl溶液的玻璃微量移液器安装在三维显微操作器上，在显微镜下穿刺细胞，当施加到电极上的电位小于50 mV时，测量系统稳定。腹膜渗出液细胞膜电位范围为-5.47 ~-24.74 mV，平均为-13.36 mV（s. D. =6.03 my）。脾细胞电位范围为-6.88 ~-14.42 mV，平均为-10.69 mV（s. D. = 8.53 my）。从GCF收集的细胞的膜电位范围为-2.2 mV至-17.6 mV，平均值为-6.4 mV。我们计划阐明兴奋的炎症细胞的膜电位与细胞功能之间的关系。

Most of the studies on inflammatory cells of acute periodontitis have been related to morphological or immunological aspects of the problem. Although the membrane potential reflects cell function, the electrophysiological method has not attract attention among investigators. The purpose of this study is to establish a method to measure the membrane potential of inflammatory cells collected from acute periodontitis and to relate the recorded potential to cell functions.To calibrate the measuring system, the intracellular potential was measured using cells collected from peritoneal exudate and spleen. Inflammatory cells were collected from human gingival crdvicular fluid(GCF)using glass miniature capillaries. Then the cells were transplanted to the MEM broth with 10% FCS and incubated for 30 to 60 minutes in 5% carbon dioxide atmosphere. Glass micropipettes filled with 3 M KC1 solution were mounted on a 3 dimensional micromanipulator and the cells were impaled under the microscope.The measuring system was stable when the potential applied to the electrode was less than 50 mV. The membrane potential of cells from peritonealexudate ranged from -5.47 mV to -24.74 mV and the mean was -13.36 mV(s. d. =6.03 my). The potential of spleen cells ranged from -6.88 mV to -14.42 mV and the mean was -10.69 mV(s. d. = 8.53 my). The membrane potential of cells collected from GCF ranged from -2.2 mV to -17.6 mV and the mean was -6.4 mV.We are planning to elucidate the relationship between the membrane potentials of excited inflammatory cells and cell function.