Immunohistochemical Analysis of Odontogenic and Osteogenic Tumors

牙源性肿瘤被认为起源于牙胚的上皮和/或间充质组织，因为这些肿瘤与牙胚在形态上有相似性。但大多数牙源性肿瘤的起源和组织发生尚未得到证实。淀粉原蛋白是釉质发育早期的一种有机基质，是一种富含脯氨酸的低分子量糖蛋白，与角蛋白或胶原蛋白分子不同（10-11）。由于Takagi等人（12）和Yeh等人（13）完成了淀粉原蛋白的完整氨基酸序列，因此开发了抗淀粉原蛋白的非交叉反应单克隆抗体。利用这些单克隆抗体，在超微结构上检测了成釉原蛋白，并阐明了晚期成釉前细胞和分泌型成釉细胞分泌成釉原蛋白的情况。检测成釉原蛋白被认为是有用的标记功能成釉细胞澄清牙源性肿瘤的组织发生。在牙源性肿瘤中研究了细胞角蛋白、淀粉原蛋白和釉素的组织化学表达；成釉细胞瘤，腺瘤样牙源性肿瘤，成釉细胞癌。本研究使用了四种抗细胞角蛋白单克隆抗体：CK-1抗总角蛋白，SE-K抗56,56.5,58和68Kd，NSE-K抗52.5Kd， 19K抗40Kd。滤泡性成釉细胞瘤的细胞角蛋白表达与牙胚上皮相似，网状成釉细胞瘤的细胞角蛋白表达与胎儿口腔黏膜相似。两种类型的成釉细胞瘤也表现出轻微的免疫反应性。在腺瘤样牙源性肿瘤中，细胞角蛋白在瘤巢中心细胞和周围细胞中的表达存在差异。此外，细胞角蛋白在腺瘤样牙源性肿瘤中的表达与成釉细胞瘤和牙胚中的表达完全不同。腺瘤样牙源性肿瘤的胶体滴、钙化团块和导管状结构的内膜对淀粉原蛋白和漆包素有免疫反应。因此，这些肿瘤细胞可能比成釉细胞瘤更容易分化为产生牙釉质蛋白的细胞。成釉细胞癌的免疫组化结果显示，该癌由未分化的牙源性肿瘤细胞组成。此外，我们还讨论了与原纤维骨相比，下颌髁突软骨中I型、II型和III型胶原定位的免疫组织化学研究。成骨肿瘤中骨基质蛋白的检测。少

Odontogenic tumors are thought to be originated from epithelial and /or mesenchymal tissue of tooth germ, because of morphological similarities between these tumors and tooth germs. But the origin and histogenesis of most of the odontogenic tumors have been remained to be unproven. Amelogenin, an organic matrix of enamel in the early developmental stage, is well known to be proline rich glycoprotein with low molecular weight, distinct from keratins or collagen molecules(10-11). Since complete amino acid sequence of amelogenin was accomplished by Takagi et al (12) and Yeh et al (13), non cross-reacting monoclonal antibodies against amelogenin were developed. With these monoclonal antibodies, amelogenin was detected ultrastructurally, and merocrine secretion of amelogenin by late preameloblasts and secretory ameloblasts was elucidates. The detection of amelogenin was considered to be useful as a marker of functioning ameloblasts in clarifying the histogenesis of odontogenic tumors.Immuno … More histochemical expressions of cytokeratin, amelogenin, and enamelin were studies in odontogenic tumors; ameloblastoma, adenomatoid odontogenic tumor, ameloblastic carcinoma. Four anti-cytokeratin monoclonal antibodies were used in this study: CK-1 to total keratin, SE-K to 56,56.5,58 and 68Kd,NSE-K to 52.5Kd,and 19K to 40Kd. Cytokeratin expression in follicular ameloblastoma was similar to that in tooth germ epithelia, and in plexiform ameloblastoma similar to that in fetal oral mucosa. Both types of ameloblastoma also showed slight immunoreactivity for amelogenin. In adenomatoid odontogenic tumor there was a difference between cytokeratin expression in central cells and in peripheral cells in the tumor nests. Moreover, cytokeratin expression in adenomatoid odontogenic tumor was completely different from those in ameloblastoma and tooth germ. Immunoreactivity for amelogenin and enamelin in adenomatoid odontogenic tumor was found in colloidal drops, calcified masses and lining cell membrane of ductlike structure. Therefore, it is possible that these tumor cells differentiate into enamel protein-producing cells, more than those of ameloblastoma do. Immunohistochemical results in ameloblastic carcinoma showed that this carcinoma is composed of undifferentiated odontogenic tumor cells. Also, an immunohistochemical study of localization of type I,II and III collagen were discussed in mandibular condylar cartilage compared with fibrillar bone. Bone matrix protein detected in osteogenic tumors. Less