Studies on stimulation of feeding behavior in yellowtail Seriola quinqueradiata

采用凝胶色谱、离子交换色谱等分析方法，研究了竹荚鱼白色肌肉水提液对青尾Seriola quinqueradiata幼鱼的促摄食作用。通过将提取物的测试级分应用于淀粉颗粒中的幼黄尾鱼并计算鱼吃掉的颗粒的数量来评估提取物的测试级分的摄食刺激效果。(1)在Sephadex-15柱上的提取物的凝胶级分将肌肉的水提取物通过滤膜（UK-15，Advantic，M.W.截断值10，000），超滤液在SephadexG-15柱上从0.05M氯化钠中分级。将获得的级分分组并合并。然后，将汇集的鱼精冷冻干燥，并将残留物溶解在海水中并施用于鱼。结果表明，有效成分主要分布在分子量几百或更低的组分中。(2)用DEAE-Sephadex A-25阴离子交换剂、（B）CM-Sephadex C-25阳离子交换剂或（c）两种离子交换剂分批处理肌肉提取物的超滤液。所获得的结果表明（a）降低了提取物的刺激效果。这表明一些有效成分被吸附到DEAE-Sephadex上;（B）对提取物的有效性没有明显影响;（c）导致有效性的严重降低，这表明吸附到阳离子交换剂上的成分也显示出与（c）处理的提取物中的成分协同的摄食刺激作用。

The feeding-stimulatory effectiveness of a water extract of the jack mackerel Trachurus japonicus white muscle for the young yellowtail Seriola quinqueradiata was studied with the aid of gel chromatography, ion exchange chromatography and other analytical methods. The feeding-stimulatory effectiveness of test fractions of the extract was evaluated by applying them to the young yellowtail in starch pellets and counting the number of pellets eaten by the fish. (1) Gel fractions of the extracts on a column of Sephadex-15 The water extract of the muscle was ultrafiltrated through a filter membrane (UK-15, Advantic, M.W. cut off 10,000) and the ultrafiltrate was fractionated on a column of Sephadex G-15 from 0.05 M sodium chloride. The fractions obtained were divided into groups and pooled. Then the pooled eluate was freeze-dried and the residues were dissolved in sea water and applied to the fish. The results showed that effective compounds were included in fractions of molecular weight of several hundreds or lower. (2) Fractions of the extracts obtained by treating with DEAE-Sephadex and CM-Sephadex The ultrafiltrate of the muscle extract was treated with (a) DEAE-Sephadex A-25 anion exchanger, (b) CM-Sephadex C-25 cation exchanger or (c) both of the two ion exchangers in batch procedures. The results obtained showed that (a) reduced the stimulatory effectiveness of the extract. This suggests that some of the effective constituents are adsorbed to the DEAE-Sephadex;(b) had no apparent influence on the effectiveness of the extract; (c) resulted in a sever depression of the effectiveness, suggesting that constituents adsorbing to the cation exchanger also show a feeding- stimulatory effect in cooperation with constituents in the (c)-treated extract.