Studies on Biosynthesis of Bioactive Compounds produced by Microalgae

通过补饲实验，研究了甲藻亚历山大藻合成聚醚大环内酯刚碘甲素的过程。通过添加[1-^&lt；13&gt；C_1]、[2-^&lt；13&gt；C_1]或[1，2-^&lt；13&gt；C_2]-醋酸钠、13&gt；C-蛋氨酸和[1-^&lt；13&gt；C_1]-葡萄糖进行饲养试验。通过一维和二维核磁共振谱的分析，归属了刚碘甲素的~&lt；13&gt；C核磁共振信号。在46个刚碘甲素碳中，40个碳(图1中的c或m)被乙酸钠标记。只有一个碳(图1中的M)也被蛋氨酸标记。用葡萄糖标记的两个碳(图1中的A和B)在核磁共振谱上显示信号强度略有增强。标记模式(图1)表明不寻常的生物合成途径不同于聚酮途径。

Biosynthesis of polyether macrolide goniodomin A, which was produced by dinoflagellate Alexandrium hiranoi, was investigated by feeding experiments. Feeding experiments were carried out by adding [1-^<13>C_1],[2-^<13>C_1], or [1,2-^<13>C_2]-sodium acetate, Methyl- ^<13>C-methionine, and [1-^<13>C_1]-glucose of cultures of Alexandrium hiranoi. ^<13>C NMR signals of goniodomin A were assigned by analyses of 1D and 2D NMR spectra. Out of 46 carbons of goniodomin A 40 carbons(c or m in Fig. 1) were labeled by sodium acetate. Only one carbon(M in Fig. 1) was also labeled by methionine. Two carbons(A and B in Fig. 1) labeled by glucose slightly showed enhancement of signal intensities in ^<13>C NMR. The labeling patterns(Fig. 1) suggested unusual biosynthetic pathway different from polyketides pathway.