Evaluation of trial denture cleansers with berberine hydrochloride

与义齿性口炎相关的义齿菌斑中念珠菌的检出率较高。为预防义齿性口炎的发生，对义齿清洁剂进行了临床试验。albicans、假丝酵母C. tropicalis和C.光滑的试戴义齿清洁剂含有盐酸小檗碱。盐酸小檗碱是抗真菌活性较强的生药成分，本身具有抗真菌活性。采用肉汤稀释法测定了几种清洁剂的杀菌活性，筛选出一种对C.对白色念珠菌进行强力清除试验，对牙用材料的颜色变化、树脂板表面粗糙度进行强力清除试验。这些试验表明，S16的念珠菌去除活性与一种商业义齿清洁剂一样，并且对物理性能没有影响。体外研究证明，试验义齿清洁剂具有抗真菌活性，并且能够从丙烯酸树脂表面去除粘附的真菌细胞。因此，使用清洁剂似乎有助于预防义齿性口炎。

Candida species can be found in high frequency in denture plaque related to denture stomatitis. It is important to clean dentures to prevent denture stomatitis.This study investigated the antifungal activity of trial denture cleansers against C. albicans, C. tropicalis and C. glabrata. Trial denture cleansers contain berberine hydrochloride. Berberine hydrochloride is a component of crude drugs which have strong antifungal activity and has antifungal activity itself. According to the broth dilution method, the fungicidal activity of cleansers was tested.One cleanser (S16) that showed activity against C. albicans strongly was tested for Candida removing from acrylic resin plates, color changing of dental materials and surface roughness of acrylic resin plates. These tests revealed that S16 Candida removing activity as well as one commercial denture cleanser and no effects on physical properties.In vitro studies proved that a trial denture cleanser had antifungal activity and ability to remove adhered fungal cells from acrylic resin surfaces. Hence, use of the cleanser seems to be avail in preventing denture stomatitis.