Liquid Chromatography-Tandem Mass Spectropmetry (LC-MS/MS)

所述项目的研究基础涵盖了与修饰和未修饰肽分析相关的广泛的定量和定性研究问题。进一步的重点是小分子代谢物的时间分辨检测。这两类分析物都注定要用于有针对性的质谱研究，对于它们明确的检测、定量和与发表相关的表征，基于三重四极杆配置的液相色谱耦合串联质谱法目前是金标准，因为它具有出色的灵敏度。该技术允许在从飞到纳克范围内超过6个数量级的动态范围内，对复杂反应混合物中的目标分子进行高度特异性、平行量化。它是特别无与伦比的动力学测量，如复杂蛋白质样品或翻译后修饰的时间分辨表达分析。质谱仪单元可与超高性能液相色谱系统或纳米液相色谱系统耦合，允许使用质谱仪进行项目相关的常规测量，以及对有限生物源材料的样品进行更具体的分析。多特蒙德大学化学和化学生物系目前还没有液相色谱联用的三重四极杆质谱仪，由于其在生物化学、化学生物学和有机化学等各个领域的广泛适用性，因此是现场可用分析仪器的必要补充

The research underlying the projects described covers a wide range of quantitative and qualitative research questions related to the analysis of modified and unmodified peptides. A further focus is the time-resolved detection of small molecule metabolites. Both classes of analytes are predestined for targeted mass spectrometric studies and for their unequivocal detection, quantification and publication-relevant characterization, liquid chromatography coupled to tandem mass spectrometry based on a triple quadrupole configuration is currently the gold standard due to ist excellent sensitivity. The technology allows the highly specific, parallel quantification of target molecules in complex reaction mixtures over a dynamic range of up to more than six orders of magnitude from the femto- to the nanogram range. It is in particular unrivalled for kinetic measurements such as time resolved expression analyses of complex protein samples or post-translational modifications. The optional coupling of the mass spectrometer unit to an ultra-high-performance liquid chromatography system or to a nano-liquid chromatography system allows the use of the mass spectrometer for project-related routine measurements as well as for more specific analyses of samples from limited biological source material. A triple quadrupole mass spectrometer coupled with liquid chromatography is currently not available at the Department of Chemistry and Chemical Biology at TU Dortmund University and, due to ist broad applicability in various areas of biochemistry, chemical biology and organic chemistry, represents an imperative addition to the analytical instrumentation available at the site