Development of Hybrid Type Artificial Bone Marrow Made of Hydroxyapatite Chamber

采用造血诱导微环境（HIM）在体内烧结羟基磷灰石（HA）腔中人工诱导骨髓组织。本研究对揭示造血机制具有重要意义。从系统发育的角度来看，已知HIM在进化的早期阶段肯定存在于最靠近消化道的器官，即脾脏。在脊椎动物的第二次革命中，内部骨骼从软骨变成了骨组织。从这一系统发生的事实来看，如果我们能找到最佳的结构条件，我们应该能够在HA腔中诱导造血巢代替骨。为了初步研究，我们进行了以下实验来观察烧结磷灰石对间质组织的组份作用：1)临床将磷灰石颗粒应用于牙髓室；2)将烧结磷灰石板置入肋骨骨髓；3)取出一段带骨髓的股骨，植入管状…型磷灰石人工骨，用a - o板固定。在一段固定的时间后，制作标本，然后进行组织病理学检查。随后，我们尝试用烧结多孔管状羟基磷灰石和高压气体技术制备的新型HA板在肌肉中人工诱导造血场。然后，我们开发了一个由烧结HA制成的人造骨髓腔，并将其连接到狗的髂动脉上。肋骨骨髓对羟基磷灰石多孔板有明显的吸收，无明显的组织反应。不论是在管状HA人工骨的孔位，还是在背肌植入的新型HA板表面，都可以观察到造血场骨髓细胞簇的分化。对植入的人工骨髓腔进行组织病理学检查。在没有骨和骨髓组织的HA骨髓腔中观察到网状内皮系统。在肌肉组织中，HA腔内可见骨髓组织成骨

Artificial inducement of bone marrow tissue with hemopoietic inductive microenvironment (HIM) in sintered hydroxyapatite (HA) chamber in vivo was carried out. This research is important to disclose the mechanisms of hemopoiesis. From a phylogenic standpoint, it is known that HIM definitely existed in the organ nearest the digestive tract, i. e., spleen, in an early stage of evolution. In the second revolution of vertebrates, the internal skeleton changed into bone tissue from cartilage. From this fact of phylogenesis, we should be able to induce hemopoietic nests in a HA chamber in place of bone, if we can find optimal structural conditions. For a preliminary study, the following experiments were carried out to observe the component effect of sintered apatite upon mesenchymal tissue : 1) apatite granules were applied to a dental pulp chamber clinically : 2) Sintered apatite plates were placed into rib bone marrow : and 3) A section of femur with marrow was removed, after which tubular- … More type apatite artificial bone was implanted and fixed with an A-O plate. After a fixed period, specimens were made and then histopathologically examined.Thereafter, we tried to induce artificially a hematopoietic field in muscles using sintered porous tubular hydroxyapatite and a new type HA plate made by a high-pressure gas technique. Then, we developed an artificial bone marrow chamber made of sintered HA,which was connected to the iliac artery of a dog. Distinct resorption of hydroxyapatite porous plates was observed in rib bone marrow without marked tissue reaction. Not only in the pore sites of tubular HA artificial bone but at the surface of the new type HA plate implanted in the dorsal muscles, differentiation of bone marrow cell clusters of the hematopoietic field could be observed.The implanted artificial bone marrow chamber was also examined histopathologically. A reticuroendothelial system was observed in the HA bone marrow chamber without bone and bone marrow tissue. In muscle tissue bone marrow tissue with bone formation in the HA chamber could be observed Less