Development of cancer imaging correlated to metastatic potential using positron emission tomography

我们已经进行了三年的实验研究，旨在利用正电子发射断层扫描技术建立与转移潜能或恶性潜能相关的肿瘤成像。为了通过测量透明质酸代谢来研究体内肿瘤的转移潜力，我们使用N-[^&lt；18&gt；F]氟乙酰-D-氨基葡萄糖([^&lt；18&gt；F]GluNFAc)对携带B16黑色素瘤变异体的C57BL/6小鼠和携带FM3A肿瘤变异体的C3H/He小鼠进行了评估。高转移变异株B16-F10对[GluNFAc]的摄取略高于低转移变异株B16-F1(p&lt；0.05)。然而，用放射自显影很难检测到每个肿瘤摄取的差异。为了利用标记氨基酸或其类似物评价体内肿瘤的恶性潜能，研究了3，4-dihydroxy-2-[^&lt；18&gt；F]fluoro-L-phenylalanine(2-[^&lt；18&gt；F]DOPA)对黑色素瘤的显影，L-2-[^&lt；18&gt；F]氟酪氨酸([^&lt；18&gt；F；F]Tyr)作为蛋白质合成的标志，L-[2-^&lt；18&gt；F]氟苯丙氨酸(FPhe)作为氨基酸运输的标志。

We have performed experimental studies for three years aiming to develop a cancer imaging correlated to metastatic potential, or malignant potential using positron emission tomography.In order to investigate the metastatic potential of tumors in vivo bymeasuring hyaluronic acid metabolism, C57BL/6 mice with B16 melanoma variants and C3H/He mice with FM3A tumor variants were evaluated using N-[^<18>F]fluoroacetyl-D-glucosamine ([^<18>F]GluNFAc) . The uptake of[^<18>F]GluNFAc was slightly higher in high-metastatic variant, B16-F10, than in low-metastatic one, B16-F1 (p<0.05) . However, it is difficult to detect the difference of each tumor uptake using autoradiography. Analysis of metabolites suggested the slower metabolism in tumors than that in liver.In order to evaluate the malignant potential of tumors in vivo using ^<18>F labeled amino acids or its analogs, 3,4-dihydroxy-2-[^<18>F]fluoro-L-phenylalanine (2-[^<18>F]DOPA) was studied for melanomas imaging, L-2-[^<18>F]fluoro-tyrosine ([^<18>F]Tyr) for a marker of protein synthesis, and L-[2-^<18>F]fluorophenylalanine ([^<18>F]FPhe) for a marker of amino acid transport.