Developmet of Clye cleavano with near-infrared tissue spectroscopy

吲哚菁绿色在近红外光区805 nm处有特定的吸光度。因此，我们已经评估了吲哚菁绿色的吸光度后，排除氧合血红蛋白和脱氧血红蛋白，氧化和还原细胞色素c氧化酶和洗出的肝脏成分的多重曲线拟合分析与组织近红外光谱。用这种新的方法，我们已经评估了组织氧合在血窦水平的红细胞血红蛋白的氧饱和度，吲哚菁绿色的跨膜转运的吲哚菁绿色管理后的增加相的速率常数，和细胞内的运输和排泄通过胆小管的速率常数的下降相，分别。胆红素作为吲哚菁绿色跨膜摄取的竞争性抑制剂，抑制给药后的增加相。秋水仙碱和哇巴因均通过破坏肝细胞微管和抑制钠钾通道抑制下降相 ...更多信息 胆管膜ATP酶活性。因此，这两个速率常数被证明是吲哚菁绿色的跨膜摄取和细胞内转运和通过胆小管排泄的指示。我们应用这种方法在肝脏缺血再灌注损伤，并表明跨膜摄取缺血再灌注损伤，但恢复迅速，而恶化的细胞内运输和排泄通过胆小管持续更长的时间。由于这些损伤与细胞内ATP减少有关，因此怀疑这些损伤是由细胞内Ca代谢恶化引起的。此外，我们使用这种方法进行肝功能检查。我们同时测量了缺血叶和对照叶的吲哚菁绿色摄取和排泄。本研究表明，非缺血叶在染料排泄方面受到对侧缺血叶的影响，表明在肝切除术中应尽可能避免缺血性损伤。少

Indocyanine green has a specific absorbance at 805nm in the near-infrared light region. Therefore, we have evaluated absorbance of indocyanine green after excluding oxy-hemoglobin and deoxyhemoglobin, oxidized and reduced cytochrome c oxidase and washed out liver components by multiple curve fitting analysis with tissue near-infrared spectroscopy. With this novel method, we have evaluated tissue oxygenation at the sinusoid level by oxygen saturation of hemoglobin of the red cells, transmembrane transport of indocyanine green by a rate constant of increase phase after indocyanine green administration, and intracellular transport and excretion through bile canaliculi by a rate constant of the decrease phase, respectively. Increase phase after administration was inhibited by bilirubin as a competitive inhibitor of transmembrane uptake of indocyanine green. Decrease phase was inhibited both by colchicine and ouabain via destruction of microtubules in the hepatocyte and suppression of Na-K … More ATPase at bile canaliculi membrane, respectively. Therefore, these two rate constants were certified to be an indicator of transmembrane uptake and intracellular transport and excretion through bile canaliculi of indocyauine green. We applied this method to ischemia reperfusion injury in the liver and showed that transmembrane uptake was injured by ischemia reperfusion injury but recovered rapidly, while deterioration of intracellular transport and excretion through bile canaliculi continued much longer. Since these injuries were related to intracellular decrease of ATP,these injuries were suspected to be caused by deterioration of intracellular Ca metabolism. Furthermore, we used this method for divided liver function tests. We have measured indocyanine green uptake and excretion in the ischemic lobe and control lobe simultaneously. This study showed that non-ischemic lobe was affected by the contralateral ischemic lobe in terms of dye excretion, indicating that ischemic insult should be avoided as less as possible in liver resection. Less