Developemt of protein crosslinking technology using the newly found transglutaminase substrate termed cementoin.

新发现的转氨酶底物，被称为Cementoin和Cementoin样部分，在大肠杆菌中使用重组DNA技术作为与麦芽糖结合蛋白的融合蛋白产生，通过亲和层析在直链淀粉树脂上纯化，并进行表征。产物容易与催化量的转氨酶交联，表明它们确实具有用作蛋白质交联剂或蛋白质共价胶的适当性质。特异性的抗血清也被生产和用于Western印迹分析，免疫组织化学染色，放射免疫分析，交联蛋白被发现是丰富的上皮的气管和肠。在银屑病患者中，牙骨质妥英/弹力素的表达显著升高。这些结果表明，牙骨质妥英及其同系物是重要的和有用的分子，不仅在蛋白质工程领域，而且在了解基础生理学。

The newly found transglutaminase substrates, termed cementoin and cementoin-like moieties, were produced in E.coli using recombinant DNA technology as fusion proteins with maltose-binding protein, purified by affinity chromatography on amyrose resin, and characterized. The products were readily cross-linked with a catalytic amount of transglutaminase, indicating that they have indeed appropriate properties to be used as a protein cross-linker or protein covalent glue. Specific antisera were also produced and used for Western blot analysis, immunohistochemical staining, and radioimmunoassay ; the cross-linker proteins were found to be rich in the epitherium of trachea and intestine. Expression of cementoin/elafin was greatly elevated in a psoriasis patient. These results indicate that cementoin and its homologues are important and useful molecules not only in the field of protein engineering but also in understanding basic physiology.