Comparative study on lymphoid malignancy between Jap。 & China -A cytogenetic and molecular biological study -

非霍奇金淋巴瘤（NHL）在日本的发病率是美国的一半。本研究旨在了解日本和印度的滤泡性淋巴瘤和慢性淋巴细胞白血病的发病率，并与西方国家的数据进行比较，同时调查这些国家在细胞遗传学和分子遗传学方面的差异。1) 73例日本NHL病例的细胞遗传学分析显示14例发病率较低；18易位在滤泡性淋巴瘤中（40%），而美国病例的发生率（75-88%）。日本CLL病例的核型异常发生率（45.8%，美国51.5%）和异常类型（12,14q和13q三体异常）与美国病例几乎相同。而日本的复杂核型发生率高于西方国家（p<0.05）。2)日本/印度滤泡性淋巴瘤的BCL-2重排发生率低于美国病例（南方印迹法为45.2%，美国报道为90%），PCR法为10.9%，美国报道为53.4%。BCL-2重排的发生率在日本和美国CLL患者之间没有差异（5.7% vs 6.8-9.3%）。3) FISH法检测CLL患者12三体和RB基因缺失，发现美国和印度患者12三体的发生率高于日本患者（30-42.9% vs 19%），而印度患者RB基因缺失（39%）与日本患者（28.6%）差异无统计学意义。总的来说，印度病例表现出与日本b细胞恶性肿瘤几乎相同的生物学特征，尽管与美国和欧洲病例存在一些差异。

The incidence of non-Hodgkin's lymphoma (NHL) in Japan is one-half of that in the USA. The present study was under taken to understand the incidence of follicular lymphoma and chronic lymphocyte leukemia in Japan and India in comparison to the data from the western countries, and also to investigate the differences among these countries in cytogenetic and molecular genetic findings.1)Cytogenetic analysis of 73 Japanese NHL cases revealed a lower incidence of 14 ; 18 translocation among follicular lymphoma (40%), compared with the incidence in American cases (75-88%). Japanese CLL cases had almost the same incidence of karyotype abnormalities (45.8%, 51.5%, in USA) and types of abnormalities as American cases (trisomy 12, 14q and 13q abnormalities).However, a higher incidence of complex karyotype was found in Japanese cases than that of western countries (p<0.05).2)The incidence of BCL-2 rearrangement in Japanese/Indian follicular lymphoma was less than that of American cases (45.2% vs 90% USA cases in reports) by Southern blotting, 10.9% vs 53.4% USA cases by PCR. The incidence of BCL-2 rearrangement was not different between that of Japanese and American CLL cases (5.7% vs 6.8-9.3%).3)Detection of trisomy 12 and RB gene loss by means of FISH method in CLL cases revealed that American and Indian cases had a higher incidence of trisomy 12 than that of Japanese cases (30-42.9% vs 19%), whereas RB gene loss of Indian cases (39%) was not significantly different from Japanese cases (28.6%). In general, Indian cases showed almost the same biological characteristics of Japanese B-cell malignancies, although there exists some differences with the American and European cases.