Regulation of Gene Expression

得到了以下结果：1。我们分离到了几个在aba介导的高等植物形态发生调控中必不可少的Vp-1或Abi - 3基因的同源物。通过基因靶向，我们研究了包括mel-18在内的几个基因的功能，mel-18是一个负调控转录因子，控制同源盒基因的表达。Mel-18基因敲除显示了骨骼的后向转化，表明它们的功能与果蝇相同。通过核磁共振分析了IgG与蛋白a、-G和-L之间的分子相互作用。结果表明：1)蛋白a和-G的igg结合结构域具有完全不同的三维结构，但与Fc上的位点有广泛的重叠；2)蛋白G使用两个完全不同的表面区域来结合Fab和Fc区域；3)蛋白L的igg结合结构域的折叠与蛋白G相似，但它们在Fab.4中结合不同的结构域。利用间接免疫荧光显微镜分析了裂殖酵母有丝分裂过程中染色质区和微管的位置和形态变化。为了开发高灵敏度的生物传感器，利用微型机械和CCD摄像系统制作了一些微生物固定化腔室填料珠的器件。该系统的电流响应与葡萄糖浓度成正比。发光的分布理论上随着固定化酶的速率和活性的降低而减小。由于被分析物诱导的酶的化学扩增，该系统具有很高的灵敏度。我们讨论了法日免疫学的未来合作，特别是在T-B合作、调节淋巴细胞发育和免疫疾病方面。合作包括信息、材料和青年科学家的交流。

Following results were obtained :1. We isolated several homologues of Vp-1 or Abi 3 genes which are essential for ABA-mediated signal transduction in the regulation of higher plant morphogenesis.2. By using gene targeting, we investigated function of several genes, including mel-18 which is a negative regulatory transcription factor and controls the expression of homeobox genes. Mel-18 knock-outs showed posterior transformation of skeletal bones, suggesting their equivalent functions to those of Drosophila.3. By NMR,molecular interactions between IgG and protein-A,-G,and -L,were analyzed. It was revealed that 1) the IgG-binding domains of protein-A and -G have quite different three-dimensional structures, but bind to sites on the Fc that overlap extensively, 2) protein G employs two quite different regions of its surface to bind to the Fab and the Fc regions, and 3) the fold of the IgG-binding domain of protein L is similar to that of protein G,but they bind to a different domain in the Fab.4. By indirect immunofluorescence microscopy, we analyzed location and morphological change of chromatin region and microtuble especially during mitosis in the fission yeast S.pombe cells.5. For development of high sensitive biosensor, some devices with the chamber packing beads immobilized with microorganisms were fabricated by micromachine and CCD camera system. The system gave current response proportional to glucose concentration. Distribution of the luminescendce gave theoretical decrease as a function of low-rate and activity of immobilized enzyme. The system has ability to be highly sensitive due to chemical amplification by the enzyme induced by analyte.6. We discussed future collaborations on France-Japan Immunology, particularly on T-B collaboration, regulation lymphocyte development, and immune diseases. Collaboration includes exchange of information, materials, and young scientists.