Studies on bioultrasonic micro-spectroscopy

生物超声微光谱学（Bio-UMS）作为一种新的研究方法有望在生物科学和医学应用领域得到发展。Bio-UMS技术可以利用VHF和UHF范围内的体和聚焦超声波在微观尺度上研究生物软硬组织的粘弹性特性。Bio-UMS的系统和方法如下：为了利用纵波获得软组织和液体的体声学特性，开发了一种工作频率为1MHz至1GHz的生物超声光谱方法和系统。以水为参比介质的超声透射对比法可以高精度地同时确定衰减、速度、阻抗和密度的频率特性。在2 ~ 500MHz范围内，对蓖麻油、硅油和蓖麻油的衰减、速度、阻抗和密度等声学特性进行了测试。在20 ~ 300MHz范围内，已经成功地对油进行了速度色散测量。首次在20 ~ 200MHz范围内同时测定了牛肝脏、心肌和脂肪的四个参数。研究表明，肝脏和心脏肌肉组织声学特性的差异可以用它们的蛋白质含量来解释。开发了一种利用横波测定声学特性的方法和系统。在5 ~ 300MHz范围内获得了甘油和硅油的剪切波特性，具有足够的精度。本文提出了一种利用超声纵波获取VHF和UHF范围内液体非线性特性的新方法和系统。在100 ~ 180MHz的基频范围内，首次测定了糖水溶液的非线性参数。研制了一种具有成像和定量测量功能的高分辨率定向点聚焦光束声显微镜系统，用于研究牙齿和骨骼的硬组织。少

Bioultrasonic micro-spectroscopy (Bio-UMS) is expected to be developed as a new methodology in the research fields of biological science and medical applications. Viscoelastic properties of biological soft and hard tissues on a microscopic scale can be investigated by Bio-UMS technology using bulk and focused ultrasonic waves in the VHF and UHF ranges. Systems and methods for Bio-UMS have been developed as follows :1. A bioultrasonic spectroscopy method and system, operating in the frequency range from 1MHz to 1GHz, has been developed in order to obtain bulk acoustic properties of soft tissues and liquids using longitudinal waves. The frequency characteristics of attenuation, velocity, and impedance, and the density can be determined simultaneously with high accuracy by an ultrasonic transmission comparison method wherein the reference medium is water.The acoustic properties of attenuation, velocity, impedance, and density of castor oil, silicone oil, and conttonseed oil have been dete … More rmined in the range 2 to 500MHz by the system. Measurements of velocity dispersion have been demonstrated successfully for the oils in the range 20 to 300MHz.Four paramenters of bovine liver, heart muscle, and fat have been determined simultaneously for the first time in the range 20 to 200MHz. It has been shown that the differences between the acoustic properties of liver and heart muscle tissues can be explained by their protein contents.2. A method and system to determine acoustic properties using shear waves has been developed. The shear wave properties of glycerol and silicone oil have been obtained in the range 5 to 300MHz with sufficient accuracy.3. A new method and system has been developed to obtain nonlinear properties of liquids in the VHF and UHF ranges using ultrasonic longitudinal waves. Nonlinear parameters have been determined for aqueous solutions of sugars in the fundamental frequency range 100 to 180MHz for the first time.4. A high resolution directional point-focus-beam acoustic microscope system with two functions of imaging and quantitative measurements has been developed to in investigate hard tissues of teeth and bones. Less