Development of Biological Ultrasonic Micro-Specroscopy System

生物超声显微光谱系统的研制

• 批准号: 63880033
• 负责人: CHUBACHI Noriyoshi
• 金额: $ 4.86万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-63880033/
• 关键词: Ultrasonic Micro-Spectroscopy; Biological Tissue Characterization; Viscoelasticity; Acoustic Microscopy; Velocity and Attenuation; Quantitative Measurements; V(z) Curve Analysis; Line-Focus-Beam Acoustic Microscope; 干渉超音波顕微鏡; 超音波マイクロスペクトロスコピーシステム

A biological ultrasonic micro-spectroscopy (Bio-UMS) system has been developed as a new research tool to characterize biological soft and hard tissues on a microscopic scale in the VHF and UHF ranges.A method has been developed for measuring the bulk acoustic properties such as velocity, attenuation, and density in reflection and transmission modes with the biological soft tissue specimen sandwiched between the parallel surfaces of fused quartz buffer rods having ZnO piezoelectric film transducers on their opposite ends. The method is an acoustic transmission line comparison method wherein the reference medium is distilled water. Techniques of precise mechanical alignment for parallelism of the two rod surfaces, movement to adjust the gap distance, and signal processing are involved in order to obtain high measurement accuracy. Results of measurements with bovine tissues of liver, heart muscle, and fat have been presented.A reflection-type acoustic microscope system for biological soft … More tissue characterization has been developed. This system operates in both amplitude and phase modes, and can measure acoustic properties, viz., velocity and attenuation, of tissues in two-dimensional color imaging, as well as at arbitrary points in an imaging area. A demonstration for dog cardiac infarcted tissues prepared from a formalin-fixed, paraffin-embedded sample has been made. Spatial hanges of acoustic properties in tissues due to pathological degeneration were quantitatively determined. A revised model for the quantitative characterization has been also proposed wherein the effects of acoustic multiple reflection and viscosity of the sample have been taken into account.Acoustic microscopy has been discussed to investigate viscoelastic properties of biological hard tissues, taking dental materials. The quantitative applications of an line-focus-beam (LFB) acoustic microscope, as well as the imaging applications of a point-focus-beam (PFB) acoustic microscope, have satisfactorily demonstrated the possibilities. In particular, the LFB acoustic microscope can be expected to play an important role as a powerful tool to solve basic and practical problems in all kinds of dental materials, including dental hard tissues of enamel and dentine, and dental porcelains and composites for repairing human teeth. Less

生物超声微光谱（Bio-UMS）系统是一种在甚高频和超高频范围内对生物软硬组织进行微观表征的新研究工具。本文提出了一种测量反射和透射模式下体积声学特性（如速度、衰减和密度）的方法，该方法将生物软组织标本夹在石英缓冲棒的平行表面之间，缓冲棒的两端装有ZnO压电薄膜换能器。该方法是一种声学传输线比较方法，其中参考介质为蒸馏水。为了获得较高的测量精度，需要采用精密机械对准两杆面平行度、移动调节间隙距离和信号处理等技术。测量结果与牛组织的肝脏，心脏肌肉和脂肪已经提出。研制了一种反射型声学显微镜系统，用于生物软组织的表征。该系统在振幅和相位两种模式下工作，可以在二维彩色成像中测量组织的声学特性，即速度和衰减，也可以在成像区域的任意点上测量。用福尔马林固定石蜡包埋的样品制备了犬心肌梗死组织。定量测定病理变性引起的组织声学特性的空间变化。还提出了一种用于定量表征的修正模型，其中考虑了声学多次反射和样品粘度的影响。以牙科材料为例，讨论了声学显微镜对生物硬组织粘弹性特性的研究。线聚焦束（LFB）声显微镜的定量应用，以及点聚焦束（PFB）声显微镜的成像应用，已经令人满意地证明了这种可能性。特别是LFB声学显微镜，可以作为解决各种牙科材料的基础和实际问题的有力工具发挥重要作用，包括牙釉质和牙本质的牙齿硬组织，以及修复人类牙齿的牙科瓷和复合材料。少

项目成果

N. Akashi: "Quantitative characterization of biological tissues by acoustic microscopy. -Effects of multiple reflection and viscosity-" J. Acoust. Soc. Jpn. Vol.45, pp.767-775 (1989).

N. Akashi：“通过声学显微镜对生物组织进行定量表征。-多次反射和粘度的影响-”J. Acoust。

S. Kasahara: "Application of acoustic microscopy in dental research." Acousyical Imaging Vol.17, pp.153-158 (1989).

S. Kasahara：“声学显微镜在牙科研究中的应用。”

H.Okawai: "Quantitaive display of acoustic properties of the biological tissue elements." Acoustical Imaging. 17. 193-201 (1989)

H.Okawai：“生物组织元素声学特性的定量显示。”

H.Okawai;M.Tanaka;N.Chubachi;F.Dunn;K.Honda: Acoustical Imaging. 17. (1989)

H.Okawai；M.Tanaka；N.Cubachi；F.Dunn；K.Honda：声学成像。

H. Okawai: "Quantitative display of acoustic properties of the biological tissue elements." Acoustical Imaging Vol.17, pp.193-201 (1989).

H. Okawai：“生物组织元素声学特性的定量显示。”