Where and how do the initial lymphatics develop?

起始淋巴管发育的酶组织化学研究：1.淋巴管的个体发育：研究了起始淋巴管和毛细血管在几个器官中的精细结构和分布，特别是摄食、呼吸等功能的发育。淋巴管再生实验：观察大鼠和猴皮肤损伤或移植后血管的酶组织化学特性。这些皮肤组织中的淋巴管和血管分别表现出较高的5‘-Nase和ALPase活性。3.阻断肠淋巴系统的作用：用光镜和电子显微镜观察胸管结扎后淋巴管的形态变化。TD结扎后，壁内和肠系膜淋巴管扩张，中央淋巴管5‘-Nase活性降低。4.淋巴管内皮细胞体外增殖：采用胶原酶消化法从猴胸导管分离内皮细胞，在含内皮细胞生长因子的培养液中培养数周。培养的血管内皮细胞5‘-Nase阳性。需要进一步检查，以确定在体外形成的管道。

Enzyme-histochemical study of the development of initial lymphatics.1.Ontogeny of iymphatic vessels : Fine structure and distribution of initial lymphatics and blood capillaries in several organs were studied with special reference to the the development of some functions of feeding, respiration et al. The lymphatic network developed according to the development of the function of the organs.2.Experiment of the regeneration of lymphatics : Enzyme-histochemical property of the vessels in the skins, which were injured or transplanted, were examined in the rat and monkey. The lymphatics and blood vessels in these skin tissues revelaed high 5'-Nase and ALPase activity respectively.3.The effect of the blockage of the lymphatic system in the intestne : The morphological changes of lymphatic vessels after thoracic duct (TD) ligation were examined using a light and electron microscope. After the TD ligation, intramural and mesenteric lymphatic vessels were distended and 5'-Nase activity of the central lymphatic vessels decreased.4.in vitro proliferation of lymphatic endotherial cells : The endotherial cells were separated from the monkey thoracic duct using collagenase digestive method and cultured in the medium with endotherial growth factor (EGF) for a few weeks. The cultured enthotheral cells were 5'-Nase-positive. Further examination is needed to ascertain the canal formation in vitro.