Measurement of intracellular signaling events induced by cell-cell interactions in a live organ.

我们试图建立一种对细胞内信号事件进行定量检测并在计算机显示器上可视化的系统。使用的材料是淋巴细胞、胸腺细胞和胸腺器官。用抗T细胞抗原受体抗体或PMA和离子霉素刺激这些细胞。刺激15min~1h后，测定细胞内游离钙离子浓度([Ca~(2+)]i)。我们可以定量检测到[Ca~(2+)]i的快速升高和持续振荡。通过使用这笔赠款获得的这些数据发表在几个期刊上。在器官培养的胸腺中出现的胸腺细胞也成功地进行了测量。该系统是一种新颖的实验检测系统，为分析活体器官的细胞内信号事件提供了可能。

We have tried to established a system, in which intracellular signaling event are quantitatively detected and visualized on the computer displays. The materials used are lymphocytes, thymocytes, and thymic organs. Those cells were atimulated with anti-T cell antigen receptor antibodies or PMA & ionomycin. The intra-cellular free calcium ion concentration ([Ca2+]i) was measured in the stimulated cells for 15 min.to 1 hour. We could detect a rapid increase and long-lasting oscillations of [Ca2+]i quantitatively. These data obtained by using this grant are published in several journals. The measurement was also succeeded in thymocytes presented in the organ cultured thymus. This assasy system is one of the novel experimental detection system, and provide a possibility for analyzing intra-cellular signaling events in live organs.