Establishment of the strain of the invertebrate animals and the analysis and conservation of its gene

无脊椎动物品种的建立：试图在日本和中国的各个地区收集濒临灭绝的文昌鱼，并在东京大学三崎海洋生物站使用人工授精方法饲养和繁殖。此外，MMBS的调查人员用科考船对海岸进行了调查，发现并捕获了3只个体，这表明该动物在日本地区仍有少量栖息，仍然存在保存该物种的可能性，对发育过程不明的Oikopleura进行了收集并在水族馆饲养了一段时间。经人工授精，胚胎保存时间较长。在系统发育上具有重要意义的动物种是原始的三倍纲（triplobrtica）。在冈山大学乌斯马多海洋研究所，重点研究了菌株的建立、采集、饲养和人工受精的方法，并尝试了在生物学领域广泛使用的实验材料海鞘（Ciona肠子海鞘）和另一种卵母细胞透明且细胞含有浓钒酸盐的海鞘种在水族馆中饲养，以供世界各地的科学家使用。在这些物种中建立菌株也是在广岛大学的Mukaijima海洋实验室进行的。基因分析：利用从海百合精子中获得的基因组DNA，建立基因文库。以花柱形成基因、sm-50基因和Hoxgenes基因为探针，利用MMBS中海百合的基因库分离出这些基因在海百合中的同源基因。日本三重大学在实验室从矢马鱼中分离出3种肌动蛋白基因，并鉴定了其在基因中的表达部分。在此基础上，研究了矢状门与其他动物门的系统发育差异。中国金涛和日本女川等地海鞘物种的同源性及基因差异及TTT mRNA多态性研究少

Establishment of the invertebrate strain : Amphioxus which is becomming extinct were tried to collect at the various area in Japan and China and the obtained animals were reared and bred using tartificial insemination at the Misaki Marine Biological Station, the Univ.of Tokyo. Furthermore, the investigators of MMBS have investigated the sea shore with research vessels and found and caught 3 individuals suggesting that a few number of the animals still inhabit in the area of Japan and possibility to preserve the species is still remains, Oikopleura of which the developmental process has been obscure was collected and reared in the aquarium for a while. Artificial insemination was conducted and the embryo could keep for a long peiods. Platyhelminthes Convoluta naikaiensis which is the primitive triplobrastica is the phylogenicallyinteresting animai species. Establishment of the strain and the method for collection, rearing and artificial fertilization is focused in the Ushimadeo Marine S … More tation, Okayama Univ. The ascidian, Ciona intestinalis, widely used esperimental material in the field of diology and another ascidian species of which oocyte is transparent and the cell has concentrated vanadate were tried to keep in the aquarium to supply them to the scientist in the world. The establishment of the strain in these species was also conducted in the Mukaijima marine Laboratory, Hiroshima University. Gene Analysis : Using genomic DNA obtained from the sperm of sea-lilies, the gene library was made. Six genes for spicle formation, sm-50 and Hoxgenes were used as probes and gene homologues of these genes in sea-lilies were isolated usingthe gene library of the sea-lilies in the MMBS.In the Mie University, three types of actin genes were isolated from sagitta rearing in the laboratory and identified its expression portion in the gene. Under the results the phylogenic difference of sagitta and othe animal phyla was investigated. The identity and difference of gene in the ascidian species Ciona intestinalis among the habitats for example Quintao in China and Onagawa in Japan with the investigation of polymorphism of mRNA in the TTT. Less