The effects of UVA irradiation on human neutrophil function

我们研究了紫外线A （UVA）辐照对人中性粒细胞功能的体外影响。采用化学发光法测定活性氧（ROS）和血清opsonic活性。用Boyden法测定趋化活性。低剂量UVA照射（0.2-0.4 J/cm^2）不影响OC1^-的产生，而高剂量UVA照射（0.6-1.0 J/cm^2）通过灭活诱导中性粒细胞膜表面ROS的信号级联来抑制OC1^-的产生。趋化活性差异无统计学意义。UVA照射可使血清opsonic活性升高。这些结果表明，低剂量的UVA不会损害中性粒细胞相关免疫系统，而UVA光疗需要更高剂量的UVA。

We investigated the in vitro effects of ultraviolet light A (UVA) irradiation on human neutrophil function. For measurements of reactive oxygen species (ROS) and serum opsonic activity, chemiluminescence method was used. Chemotactic activity was determined by Boyden technique. UVA irradiation at low dose of UVA (0.2-0.4 J/cm^2) did not affect the generation of OC1^-, whereas high dose of UVA (0.6-1.0 J/cm^2) suppressed its production by inactivating the signalling cascade which induce ROS on the membrane surface of neutrophils. No significant difference was observed in the chemotactic activity. The serum opsonic activity was increased by UVA irradiation. These results suggest that low dose of UVA does not impair the neutrophil-related immune system, and that higher dose of UVA is required for UVA phototherapy.