Detection of Oncogenes Affecting Genetic Diagnosis and Prognosis in the Patients with Oral Cancer by CGH

1.为了寻找和定位口腔鳞状细胞癌中染色体扩增和缺失区域，我们对17个口腔鳞状细胞癌细胞系进行了CGH，并与Southern杂交结果进行了比较。在8 q22 -26、3q 25 -27、7 p12、11 q13、13 q33、14 q、15 q和20 q12上观察到重复扩增位点。缺失见于3 p、8 p、5 q21 -22、7 q31和18 q21。CGH法和Southern杂交法检测到的扩增具有较强的一致性.当比较原始肿瘤和细胞系时，它们的轮廓基本相似，只有一个例外。此外，在细胞系中没有通常发生变化的区域，但在原始肿瘤中没有，这表明在细胞系中观察到的DNA拷贝数变化主要代表原始肿瘤的DNA拷贝数变化。我们将临床和组织病理学结果与肿瘤DNA拷贝数变化相关联。WHO Ⅱ级和Ⅲ级组染色体6p 23 -25和8 q24的增加，而染色体11 p11 -13和18 q21的缺失。DNA拷贝数变化与TNM分期的比较提示染色体1 p36和10 q25 -26的缺失可能与肿瘤进展有关。

1. To find and map chromosomal regions with amplifications and deletions in oral cancsers by CGH,we performed CGH on 17 oral squamous cell carcinomas cell lines and compared to those obtained by Southern hybridization. Recurrent loci for amplification were observed on 8q22-26,3q25-27,7p12,11q13,13q33,14q, 15q and 20q12. Deletions were seen on 3p, 8p, 5q21-22,7q31 and 18q21. There was a strong concordance for amplification detected by the CGH and Southern hybridization methods.2. When the original tumors and the cell lines were compared, their profiles were essestialy similar with one exception. Further, there was no region that commonly changed in the cell lines, but not in the original tumors, suggesting that the DNA copy number changes observed in the cell lines mostly represent those of the original tumors.3. We correlated clinical and histopathological results to tomor DNA copy number changes. The increases on chromosomes 6p23-25 and 8q24 were specificaly observed in the groups of WHO Grade II and Grade III,whereas the deletions on chromosomes 11p11-13 and 18q21 observed in the same groups. Comparison of DNA copy number changes to TNM classification indicated that the deletions on chromosomes 1p36 and 10q25-26 might be related to tumor progression.