INVESTIGATION ABOUT THE MECHANISM OF RETINAL ADHESION AND ITS BREAKDOWN AND RECOVERY

1兔视网膜色素上皮机械性切除后的愈合过程通过将盐水注入视网膜下腔在兔眼中制造圆顶形视网膜脱离。取离体视网膜下直径约1.5mm的视网膜色素上皮（RPE），从形态学上观察其愈合过程。损伤后不久，伤口周围的RPE细胞开始通过滑动和迁移填充缺损区域，2周后伤口被完全覆盖。在感觉视网膜覆盖的眼，再生的RPE细胞形成类似于正常RPE的单层，微绒毛和RPE-感光细胞外节界面重组。无感觉视网膜的眼，再生的RPE呈多层结构，微绒毛短而排列紊乱。在许多Bruck膜受损的情况下，脉络膜新生血管被破坏。2兔眼视网膜裂孔的封闭过程在兔眼上制造直径为0.5mm的视网膜裂孔。制作人工玻璃体后脱离后，行玻璃体切割及玻璃体内气体填充术。术后1周，肉眼观察，所有眼的视网膜裂孔均完全闭合，组织学检查，裂孔内充满胶质细胞，二胺黄染色显示，胶质细胞在视网膜损伤处活跃地聚集，这一过程对封闭裂孔起重要作用。3类固醇对视网膜下液体吸收的影响兔子的眼睛初步结果显示，应用激素组的浆液性脱离存活时间较未应用激素组长。

1 Healing process of the rabbit retinal pigment epithelium after mechanical removal A dome shaped retinal detachment was created in the rabbit eye by injecting saline into the subretinal space. RPE under the detached retina was removed from Bruck's membrane about 1.5mm in diameter and the healing process was observed morphologically. Shortly after the injury, RPE cells around the wound started to fill the defected area by sliding and migrating, and after 2 weeks the wound was completely covered. In eyes with overlying sensory retina, regenerated RPE cells formed a monolayr similar to normal RPE.The microvilli and RPE-photoreceptor outer segment interface was reorganized. In eyes with no sensory retina, the regenerated RPE were multilayred and the microvilli were short and disorganized. In many cases of damaged Bruck's membrane, choroidal neovascularization was observed.2 Sealing process of the retinal hole in the rabbit eyeRetinal hole of 0.5mm in diameter was made in the rabbit eye. After making artificial posterior vitreous detachment, vitrectomy and gas tamponade were performed. One week after surgery, the retinal hole was perfectly closed in all eyes macroscopically.Histologically, the hole was filled with glial cells, fibroblast and RPE.Diaminido yellow staining revealed that glial cells accumulated actively to the retinal wound and this process playd important roll to seal the hole.3 Effects of steroid on the subretinal fluid resorptionExperimental serous detachment was made by injecting rose bengal and mild photocoagulation in the rabbit eye. Preliminary results showed the tendency that in a group of administrating steroid, the serous detachment survived longer than that in non-administered group.