Development of avian component vaccine with built-in adjuvanting potencial.

该项目的目的是开发一种本身具有佐剂潜力的成分疫苗。添加佐剂特性抗原的策略是使用从人工连接的基因结构翻译的重组蛋白。重点研究了肉毒杆菌毒素重链的粘附性。虽然黏附能力位于重链的C-末端一半，但对整个重链的黏附能力进行了测试。组份疫苗抗原选自禽流感病毒、新城疫病毒、土耳其鼻气管炎病毒和马立克氏病病毒。此外，我们还将MDV gB作为调节能力的首选试验。为获得重组融合蛋白(BTX-GB)，构建了BTX-GB基因片段，并与原核表达载体pET32连接。将重组蛋白注射到实验鸡体内，诱导产生抗体。而注射重组GB后的抗GB抗体浓度并不低于注射重组融合蛋白BTX-gB后的抗GB抗体和抗GB抗体的浓度，说明BTX-GB融合蛋白没有足够的佐剂作用。应该对施工进行测试。

The aim of this project is the development of a component vaccine which have adjuvanting potential in itself. The strategy for adding adjuvanting property antigens is the use of recombinant protein translated from artificially joined construct of genes. We focused the adhesive property of the heavy chain of Botulinum toxin. Although the adhesion ability indicated to be located on the C-terminal half of the heavy chain, whole sized heavy chain was tested for adhesion ability. Antigen for component vaccine was selected from avian influenza, Newcastle disease virus, Turkey rhinotrachitis and Marek's disease virus. Additionally, we determined MDV gB as the first triat for adjuvanting ability. To obtain recombinant fusion protein (Btx-gB), we construced the gene fragment and ligated with E.coli-expression vector pET32. Antibody production was induced by injections of recombinant proteins into experimental chickens. While, the concentration of anti-gB antibody after injection of recombinant gB was not lower than that of anti-gB andtibody after injection of recombinant-fusion protein Btx-gB.This data indiceated that the Btx-gB fusion protein did not have enough adjuvanting property. Anothr construction should be tested.