ESTABLISHMENT OF THE DEVELOPING AND SUPPLING SYSTEMS FOR NEW ANIMAL,DISEASE MODELS FROM SUBSPECIES OF THE MOUSE

(1)一种新的菲律宾野鼠非胰岛素依赖型糖尿病动物模型：1)腹膜糖耐量试验(IGT)显示雄性小鼠的糖耐量受损发生率高于雌性小鼠。在被维护的品系中，IGT的发病率在男性中为-50%。在F4和F5个体之间，IGT的严重程度似乎没有增加。2)IGT小鼠的胰岛素分泌能力受损，对葡萄糖刺激的反应较差。对照组小鼠(C57BL/6J)注射葡萄糖后胰岛素增加2.5倍(1.37 mg/dl至2.62 mg/dl)。然而，IGT雄性小鼠在刺激后0.5h血清胰岛素水平略有上升。3)IGT小鼠的甘油三酯(TG)水平略高于对照组，但由于样本采集是在喂养状态下进行的，因此甘油三酯(TG)水平变化幅度较大。TG水平随着采样状态(禁食/非禁食)的不同而变化。另一方面，小鼠的总核固醇值普遍较高(174-250 mg/dl)，是对照组(132 mg/dl)的两倍。(2)在菲律宾野鼠中发现了一个突变体，这些小鼠在恒定的黑暗条件下没有节律的昼夜活动。该性状受一对近100%外显性的隐性基因控制。

(1) A new animal model for non-insulin dependent diabetes mellitus from Philippine wild mouse, Mus musculus castaneus1) Intraperitoneal glucose tolerance test (IGT) showed that the males showed a higher incidence of impared glucose tolerance than females. The incidence of IGT was -50% in males among the lines being maintained. There seems to be no increase in the degree of severity in IGT between F4 and F5 individuals.2) Insulin secretory capacity of IGT mice was impaired with poor response to glucose stimulation. The control mice (C57BL/6J) showed a 2.5 times increase in insulin upon glucose injection (1.37mg/dl to 2.62mg/dl). However, there was small rise in serum insulin 0.5 h after stimulation in the IGT males.3) Triglyceride (TG) levels in the IGT mice was slightly higher than that in the control mice although it showed a large range in variation since sample collection was done in the fed state. The TG levels vary with the state (fasted/nonfaste) of sampling. On the other hand, total coresterol (TC) levels in the mice was generally higher (174-250 mg/dl) with values twicw that of the control mice (132 mg/dl).(2) A mutant was found in the Philippine wild mice, those mice exhibit no rhythmic circadian activity under the constant dark condition. The trait was found to be controled by a recessive gene with nearly 100% penetrance.