Experimental Pathological Evaluation on Laboratory Suncus for Tonsillar Research

在临床病理上，扁桃体是粘膜免疫的重要诱导部位。然而，由于目前常规的实验动物小鼠、大鼠和豚鼠都缺乏扁桃体，扁桃体的实验研究很少，在这种情况下，本研究选择了一种新的实验动物--臭鼩（Suncus Murinus）。这种动物有一对腭扁桃体和输卵管扁桃体。单个扁桃体由隐窝样淋巴上皮共生和单个淋巴结组成。它们的隐窝上皮在管腔部位含有M细胞。毛细血管后微静脉和传出神经位于边缘区。免疫组化结果显示，T细胞分布于滤泡旁带，B细胞分布于滤泡内。在鼓室内辣根过氧化物酶（HRP）致敏中，不仅在输卵管扁桃体中检测到大量HRP抗体阳性B细胞，而且在其区域淋巴结、脾脏和腭扁桃体中检测到很少的HRP抗体阳性细胞。虽然目前还不清楚HRP抗体是IgG型还是伊加型，但M细胞的存在和系膜区HRP抗体的沉积表明HRP抗体部分含有伊加型。本研究结果支持悬雍垂对了解扁桃体区域免疫有一定的价值。本实验室正在研制抗向日葵伊加抗体的单克隆抗体。伊加单克隆抗体将为扁桃体病理学的研究提供更有意义的信息。

Clinico-pathologically, human tonsils have been marked as an important iducer site of mucosal immunity. Neverthless, the tonnsillar laboratory investigations are very little because the present conventional laboratory animals ; mice, rats and guinea pigs lack tonsils.Under such conditions, a new laboratory animal, Suncus (Suncus Murinus) was selected in this study. The animal has a pair of palatine and tubal tonsils. The individual tonsils consisted of crypt-like lymphoepithelialsymbiosis and single lymph nodules. Their crypt-epithelia contained M cells in the luminal sites. The postcapillary venules and efferent lymphatics localized in the marginal regons. Immunohistochemical preparations stained by our developed anti-Suncus T and B cell monoclonal antibodies revealed that T cells occupied the parafollicular zone and B ells located within the follicles. In the intra-tympanic cavity horseradish peroxidase (HRP) sensitization, numerous HRP-antibody positive B cells were detected not only in the tubal tonsil but rarely in their regional lymph node, spleen and palatine tonsil. Although it is unclear whether the HRP-antibody consists of IgG class or IgA,the presence of M cells and the mesangial HRP-antibody deposition pressume that the antibody partially contain IgA ones. The present study supports that the suncus is worthy for understanding tonsillar regional immunity. We are trying to develope anti-Suncus IgA antibody monoclonal antibody. The IgA monoclonal antibody will bring us further significant information in the tonsil pathology.