Development of Diagnosis System for Atherosclerosis Based on the Autofluorescence of Blood and Vascular Walls

本实验研究了豚鼠动脉粥样硬化模型和人动脉粥样硬化患者的血液和血管壁的自体荧光之间的关系，得到以下结果：1.人动脉粥样硬化主动脉的自体荧光中含有波长比正常主动脉长的成分，提示通过玻璃纤维导管进入血管系统进行光谱分析诊断动脉粥样硬化主动脉壁是可能的。2.动脉粥样硬化患者血浆中的自体荧光峰波长比正常人血浆中的自体荧光峰波长短。这一现象主要是由于血浆中脂蛋白、低密度脂蛋白（LDL）和高密度脂蛋白（HDL）的氧化所致。3.豚鼠动脉粥样硬化模型在胆固醇水平和脂质存款在动脉壁的定位等方面与人类动脉粥样硬化非常相似，为动脉粥样硬化的研究提供了一种新的方法。对豚鼠血浆、红细胞和白色细胞进行自体荧光分析。胆固醇组豚鼠血浆自发荧光强度较强，峰值波长较短，与人血浆相似。红细胞和白色细胞的自体荧光随胆固醇升高而增加。特别是红细胞的自发荧光物质被证实是原卟啉。此外，我们测量了主动脉嗜苏丹面积，发现该面积与血浆和红细胞的自体荧光强度有显著相关性。

We have performed experiments to examine the relationships between autofluorescence of blood and vascular walls of guinea pig atherosclerotic model and human patients and obtained the following results.1.The autofluorescence from human atherosclerotic aorta included the components with longer wave length than normal aorta, suggesting that diagnosis of atherosclerotic aortic walls will be possible using spectroscopic analysis through glass fiber catheter into vascular system. Further studies should be needed to the quantitative diagnosis.2.The autofluorescence from blood plasma of human atherosclerotic patients has showed that the peak wave length was shorter than that of normal plasma. This phenomenon was mainly caused by the oxidization of plasma, especially lipoproteins, low density lipoproteins (LDL) and high density lipoproteins (HDL) .3.Atherosclerotic model of the guinea pigs was quite similar to human atherosclerosis at the points of cholesterol levels and localization of lipid deposit to arterial walls, and showed to be useful for the studies of atherosclerosis. Autofluorescence analysis on blood plasma, red cells and white cells of guinea pigs was performed. Plasma of cholesterol-fed guinea pig had stronger autofluorescence with shorter wavelength at the peak, being similar to human plasma. Autofluorescence from red and white cells increased with cholesterol. Especially the autofluorescence materials from red cells was confirmed to be protoporpnyrin. Further, we measured sudanophilic area in aorta and found that the area had significant correlation with the strength of autofluorescence from plasma and red cells.