ISOLATION AND CHARACTERIZATION OF CYTOKINES SECRETED FROM CARDIAC MYXOMAS AND APPLICATION FOR CONGESTIVE HEART FAILURE

我们研究了心脏粘液瘤分泌的细胞因子的结构和特性，并分析了这些细胞因子在充血性心力衰竭中的作用。18例心脏粘液瘤中，15例（83%）抗白细胞介素6（IL-6）抗体阳性。IL-6阳性的心脏粘液瘤患者表现为自身免疫样症状，如消瘦、易疲劳、发热等。他们的实验室检查结果显示，免疫球蛋白G和A或γ球蛋白水平较高。用Western blotting分析心脏粘液瘤分泌的细胞因子。分子量为约10至12 kDa。用抗白细胞介素-8（IL-8）抗体检测此条带呈阳性。我们比较了IL-6和IL-8对病毒性心肌炎的作用。IL-6对小鼠病毒性心肌炎有治疗作用，而IL-8对小鼠病毒性心肌炎无治疗作用。组织学检查和病毒分析显示IL-6可减轻心肌坏死。因此，我们推测心脏粘液瘤分泌的IL-6可诱导心脏粘液瘤患者的自身免疫样症状，IL-6对EMCV诱导的小鼠心脏粘液瘤的减轻有一定的作用。

We investigated the structure and charactrization of cytokines secreted from cardiac myxomas and analyzed the role of these cytokines for cangestive heart failure. Among eighteen cases of cardiac myxomas, 15 cases (83%) wass positively stained with antiinterleukin-6 (IL-6) antibody. Patients with IL-6 positive cardiac myxomas showed autoimmune like symptoms, such as weghtoloss, easy fatigability and fever. Their laboratory findings revealed higher levels of immunoglobulin G and A or gamma-globulin. Cytokines secreted from cardiac myxoma was analyzed by Western blotting. The molecular weight was about 10 to 12 kDa. This band was positive with anti-interleukin-8 (IL-8) antibodies.Cultured supernatants obtained from cardiac myxomas inproved the survival of mice infected with encephalomyocarditis virus (EMCV). We compared the effects for viral myocarditis inbetween IL-6 and IL-8. IL-6, not IL-8, was beneficial for viral myocarditis of mice. Histopathological examination and viral analysis showed the reduction of myocarial necrosis by IL-6.Therefore, we hypohtesize that IL-6 secreted from cardiac myxoma induces autoimmune like symptoms in patients with cardiac myxoma and that IL-6 is beneficial for the reduction of cardiac myxomas of mice induced from EMCV.