A STUDY OF GENETHERAPY AGAINST ORAL CANCER TARGETTED TO THE GENE OF AUTOCRINE GROWTH FACTOR

成纤维细胞生长因子-1(成纤维细胞生长因子-1)和成纤维细胞生长因子-2是广谱有丝分裂原。本研究采用免疫组织化学方法检测了恶性涎腺肿瘤和正常涎腺组织中成纤维细胞生长因子-1、成纤维细胞生长因子-2及其受体FGFR-1的表达。在7例腺样囊性癌(ACC)中，导管样细胞和改良肌上皮细胞均呈阳性表达。在5例粘液表皮样癌(MC)中，表皮样细胞、粘液细胞和中间细胞3种类型的肿瘤细胞均表达免疫反应阳性的FGF1、FGFR2和FGFR 1。在这些恶性涎腺肿瘤中，FGFR-1的表达增加与成纤维细胞生长因子-2和成纤维细胞生长因子-2的免疫反应强度有关。与涎腺恶性肿瘤相比，8例正常涎腺呈阴性表达，4例呈阳性表达，提示涎腺恶性肿瘤较正常涎腺过度表达，提示这些生长因子可能在涎腺肿瘤的发生发展中起重要作用。

Fibroblast growth factor-1 (FGF-1) and FGF-2 are broad spectrum mitogens. Here, th expression of FGF-1, FGF-2 and their receptor, FGF receptor-1 (FGFR-1) was examined i malignant salivary gland tumors and normal salivary glands using immunohistochemical methods. I seven cases of adenoid cystic carcinoma (ACC), both duct-like cells and modified myoepithelial cell were apparently immunopositive for FGF-1, FGF-2 and FGFR-1. In five cases of mucoepidermoi carcinoma (MC), all three types of tumor cells including epidermoid cells, mucous cells an intermediate cells expressed immunoreactive FGF-1, FGF-2 and FGFR-1. In these malignant salivar gland tumors, increased expression of FGFR-1 correlated with the intensity of both FGF- and FGF-2 mmunoreactivity. In contrast to malignant salivary gland tumors, eight cases of normal salivary glan howed negative immunostaining for FGF-1, FGF-2 and FGFR-1 while four cases were wea immunoreactivity for FGF and its receptor.These results demonstrated that malignant salivary gland tumors overexpress FGF-1, FGF-2 an GFR-1 compared to normal salivary glands, suggesting that these growth factors may play a mportant role in facilitating its neoplastic progression in human salivary gland.