Cell kinetics in cultured tumor cells aftertreatment with radiation and chemotherapy

用抗溴脱氧尿苷（BrDU）单克隆抗体和碘化丙啶（PI）双重染色分析细胞周期。照射后的细胞动力学的变化进行了比较，与传统的PI为基础的DNA直方图方法所看到的。辐射对细胞动力学的影响主要通过计数G2期阻滞细胞来研究。用BrdU法观察到，在辐照后2 h内，G_1期迅速转变为S期，随后出现G_1期阻滞。处于S期的细胞进展到G2+M，在那里它们停滞，导致S细胞百分比降低（<5%）。> 18 h后释放G1。首次用BrdU-PI双标记法证实了辐射诱导的初始Glarrest。

The cell cycle was analyzed using double staining with an anti-bromodeoxyuridine (BrDU) monoclonal anti-body and propidium iodide (PI). Changes in cell kinetics after irradiation were compared with those seen by the conventional PI-based DNA histogram method. The effect of irradiation on cell kinetics has been studied primarily by counting G2 arrested cells. By the present BrdU method, a rapid tradition, from G1 to S-phase was observed within 2h of irradiation, followed by G1 blook. Cell in the S-phase progressed to G2+M where they arrested, resulting in a decreased percentage of S cells, (<5%). Release of G1 after>18h. The initial Glarrest induced by irradiation was confirmed for the first time by the present BrdU-PI double atsining.