Diagnpsis of lung cancer with human monoclonal antibody

制备了一种抗人肺癌单克隆抗体，用于肿瘤的治疗和诊断。1995年，利用手术切除的20例人肺癌组织标本，对该抗体进行FITC标记，检验其对人肺癌细胞的特异性。对人肺鳞癌细胞和小细胞癌细胞具有特异性亲和力。用这些癌组织的肉末进行检查也得到了同样的结果。然而，该抗体的亲和力不足以获得良好的癌组织染色。虽然每个癌组织的某些部分确实被染色，但即使在这些部分，其程度也不令人满意。该单克隆抗体的另一项研究是用肺癌和炎症性肺病患者和健康志愿者排出的痰液进行的。肺癌患者的痰液在病理学上已被证实含有癌细胞。该抗体仅对鳞状细胞癌患者痰液中含有的癌细胞进行染色。这一数据表明该抗体具有良好的特异性。现在需要建立一种新的细胞系，能够产生更多的人单克隆抗体，对癌细胞有更强的亲和力。我们从1996年开始获得这样的细胞系。

A human monoclonal antibody against human lung cancer was prepared to use for cancer treatment and diagnosis.In 1995, this antibody was labeled with FITC and inspected its specificity for human lung cancer cells by using 20 specimens of human lung cancer tissues resected in the operations. This examination proved it had specific affinity to human squamous cell carcinoma cells and small cell carcinoma cells of the lung. The same result was obtained in the examination using the mince of these cancer tissues. However, this antibody's affinity was insufficient to get well stained cancer tissues. Though some parts of each cancer tissue were certainly stained, even in such portions their degree were not satisfying.Another study for this monoclonal antibody was tried with the sputa discharged from the patients with lung cencer and inflammatory lung diseases and from healthy voluntiers. The sputa from lung cancer patients had been previously certified to include cancer cells in themselves pathologically. Only the cancer cells contained by sputa from the patients with squamous cell carcinoma were stained by this antibody. This data shows the good specificity of this antibody.Now it is required to establish a new cell line which can produce more amount of human monoclonal antibody with the stronger affinity for cancer cells. We started to get such a cell lines in 1996.