MOLECULAR BIOLOGIC STUDY ON CORTICOSTEROID-INDUCED GLAUCOMA

本实验采用日本大耳白兔右眼眼球筋膜下注射曲安奈德20或40 mg，连续4周定期测量眼压。用阿辛蓝或铜光蓝对动物前房角组织进行组织学和组织化学检查。与对照左眼相比，眼内压没有显著增加。皮质类固醇注射眼的小梁组织中未检测到组织学或组织化学变化。结果提示，人眼和兔眼对皮质类固醇的反应性可能存在一定差异，本文探讨了眼前节缺血对眼前节血管生成的影响。我们烧灼了14只家兔右眼的两条睫状后长动脉。分别于烧灼后1、2、4、7、9、14 d摘除眼球，光镜观察。血管内皮细胞的定位 ...更多信息 检测组织中VEGF蛋白及VEGF mRNA的表达。在虹膜前表面和小梁网中检测到新生血管。在视网膜中未观察到组织学变化。VEGF蛋白在虹膜上皮细胞和血管细胞中表达。睫状体上皮细胞表达VEGF mRNA。本文对4例剥脱综合征患者眼球摘除后的小梁组织进行了组织学和免疫组化检查。剥脱纤维被揭示含有硫酸软骨素硫酸皮肤素和硫酸乙酰肝素型蛋白多糖以及HNK-1表位，而细胞外基质中的微纤维没有。结果表明，蛋白聚糖和其他大分子在微纤维上的沉积可能与脱落物质的形成密切相关。我们从形态学上检查了摘除的人眼（包括早产儿和老年受试者）的睫状体带宽度与后利姆布斯宽度的比率，称为理论C/L比率。我们还在临床上检查了前房角镜检查中虹膜角膜角开放的青光眼患者和年龄匹配的对照组的睫状体带外观。理论C/L比为1.4（± SY）。[)2.7早产儿占24.0（±）SY. [)5.4儿童36.7%，[)8.0%的老年人。前房角镜检查，77.8%的青光眼和13.8%的对照眼显示睫状体带不可见或非常狭窄。结果表明，睫状体带看不见或很窄，代表睫状体角发育不全。少

We performed subtenon injection of 20 or 40 mg triamcinolone acetonide into right eyes of Japanese rabbits, and measured their intraocular pressures periodically for 4 weeks. The anterior chamber angle tissues obtained from the animals were examined histologically and histochemically using alcian blue or cupromeronic Blue. There was no significant increase of the intraocular pressure compared to control left eyes. No histological or histochemical change was detected in the trabecular tissues from corticosteroid injected eyes. The results suggest that there may be some difference in reactivity to corticosteroid between human and rabbit eyes.The effects of anterior segment ischemia on angiogenesis in the anterior segment of the eye were investigated. We cauterized both of the long posterior ciliary arteries in the right eye of 14 rabbits. The eyes were enucleated 1,2,4,7,9 and 14 days after cauterization and were studied with light microscopy. The localization of vascular endothelial gro … More wth factor (VEGF) protein and the expression of VEGF mRNA in the tissues were studied. Neovascularization on the anterior surface of the iris and in the trabecular meshwork was detected. No histological changes were observed in the retina. VEGF protein was detected in the epithelial and vascular cells of the iris. Expression of VEGF mRNA was detected in the epithelial cells of the ciliary body. These results suggest that anterior segment ischemia causes neovascularization in the anterior segment of the eye.The trabecular tissues from 4 enucleated human eyes with exfoliation syndrome were examined histologically and immunohistochemically. Exfoliation fibers were revealed to contain chondroitin sulfate dermatan sulfate and heparan sulfate-type proteoglycans as well as HNK-I epitope, while microfibrils in the extracellular matrix did not. The results suggest that depositions of proteoglycans and other macromolecules on the microfibrils may be closely associated with the formation of exfoliation materials.We examined morphologically the ratio of the ciliary body band width to the width of the posterior limbus, termed the theoretical C/L ratio, of enucleated human eyes, including those from premature infants as well as from older subjects. We also examined clinically the appearance of the ciliary body band on gonioscopy in patients having glaucoma with open iridocorneal angles, and in age mathched controls. The theoretical C/L ratio was 1.4(]SY.+-。[)2.7% in premature infants, 24.0(]SY.+-。[)5.4% in children and 36.7(]SY.+-。[)8.0% in the older subjects. On gonioscopy, 77.8% glaucomatous eyes and 13.8% control eyes showed either an invisible or very narrow ciliary body band. The results indicate that an invisible or very narrow ciliary body band represents an nuderdevelopment of the angle. Less