Experimental study on multidisciplinary treatment for malignant tumors of the eye.

本研究的目的是发展多学科的治疗方法，包括电化学疗法、光动力学疗法和热疗。将其中一些样品单独暴露于不同强度的电脉冲（500-2500）V/cm）。然后将其他细胞暴露于抗增殖剂30分钟：博来霉素、丝裂霉素C或5-氟尿嘧啶，有或没有电脉冲。通过第3天的细胞计数和H^3-胸苷摄取试验评估细胞增殖。仅在2500 V/cm下观察到细胞数量的显著减少。博莱霉素、丝裂霉素C和5-氟尿嘧啶处理无论有无电脉冲都以剂量依赖的方式抑制细胞增殖。电脉冲处理诱导的博莱霉素细胞增殖抑制作用比单用博莱霉素显著100倍。将培养的恶性黑素瘤细胞移植到小鼠眼睑中。待移植瘤细胞生长后，采用电穿孔法进行电化学治疗。所用药物为博来霉素、丝裂霉素C和5-氟尿嘧啶。电化学疗法中最有效的药物是博莱霉素，因此电化学疗法可以减少治疗恶性肿瘤所需的抗增殖药物的剂量。我们为青光眼手术开发了这种电化学疗法。

The purpose of this study is to develop multidisciplinary treatments in ocular tumors, using electrochemotherapy, photodynamic therapy and hyperthermic treatment.Malignant melanoma cells were cultured. Some of these sells were exposed to various intensities of electric pulse alone (500-2500)V/cm). Other cells were then exposed for 30 min to an antiproliferative agent : bleomycin, mitomycin C or 5-fluorouracil with or without electric pulse. Cell proliferation was assessed by cell counting on day 3 and by a H^3-thymidine uptake assay. A significant reduction in the cell number was observed only at 2500V/cm. Bleomycin, mitomycin C and 5-fluorouracil treatment inhibited cell proliferation in a dose-dependent manner either with or without electric pulse. Electric pulse treatment induced an inhibitory effect of bleomycin cell proliferation which was 100 times more prominent than bleomycin alone..Malignant melanoma cells cultured were transplanted into the eyelid of the mouse. After the growth of the transplanted tumor cells, electrochemotherapy using electroporation method was performed. The drugs used were bleomycin, mitomycin C and 5-fluorauracil. The most effective drug is bleomycin for this electrochemotherapy.The electrochemotherapy may therefore reduce the necessary dose of antiproliferative agents for the treatment against malignant tumors. We developed this electrochemotherapy for glaucoma surgery.