The relationship between neural substances (calcium-binding proteins and neuropeptides) and inflammation in the tooth pulp and salivary gland

大鼠三叉神经节含有丰富的calbindin D-28k (CB) -或S100-ir免疫表达（ir）神经元。这些神经元大多很大，分布在整个神经节中。双免疫荧光法显示这些钙结合蛋白（CaBPs）、calretinin （CR）和parvalbumin （PV）共存。在大鼠臼齿髓中观察到cb -或S100-ir神经纤维。S100-ir定位于雪旺细胞、无髓鞘和有髓鞘轴突，而CB-ir定位于有髓鞘轴突。逆行示踪和免疫组织化学方法表明，CB-或S100-ir神经纤维起源于三叉神经节。牙髓原代神经元对CB和S100分别有23%和50%的免疫反应。在给药后观察CaBP-或神经肽-ir神经纤维分布的变化。正常下颌下管周围神经纤维中可见CR-、P物质(SP) -或降钙素基因相关肽(CGRP) -ir。CR-ir神经纤维共表达SP-和cgrp -ir。注射CFA后5 d，炎症的下颌下管周围CR-ir消失，而CGRP-或SP-ir保持不变。三叉神经节石蜡切片、固定冷冻切片和新鲜冷冻切片检测CR mRNA表达。石蜡切片含有CR mRNA的信号，而固定或新鲜冷冻切片则不含。该信号主要在大神经元中观察到。这与之前CR的免疫组织化学数据一致。

The rat trigeminal ganglion contained abundant calbindin D-28k (CB) - or S100-ir immunorecative (ir) neurons. These neurons are mostly large and distributed throughout the ganglion. The double immunofluorescence method revealed the coexistence of these calcium-binding proteins (CaBPs), calretinin (CR) and parvalbumin (PV). CB-or S100-ir nerve fibers were observed in the molar tooth pulp of the rat. S100-ir was localized to Schwann cells, and unmyelinated and myelinated axons, whereas CB-ir was to myelinated axons. The retrograde tracing and immunohistochemical methods demonstrated that CB- or S100-ir nerve fibers originated from the trigeminal ganglion. 23 % and 50 % of tooth pulp primary neurons were immunoreactive for CB and S100, respectively.The change in the distirution of CaBP- or neuropeptide-ir nerve fibers was examined after administration of 10 micro 1 of complete freund adjuvant (CFA) to the submandibular ducts. CR-, substance P (SP) - or and calcitonin gene-related peptide (CGRP) -ir was observed in nerve fibers around the normal submandibular duct. The CR-ir nerve fibers coexpressed SP- and CGRP-irs. 5 days after the injection of CFA,CR-ir disappeared around the inflamed submandibular duct, while CGRP- or SP-ir ones remained unchanged.CR mRNA was examined in paraffin, fixed frozen and fresh frozen sections of the trigeminal ganglion. The paraffin sections contained the signals for CR mRNA,while fixed or fresh frozen sections were devoid of them. The signal were mostly observed in large neurons. This was consistent with the previous immunohistochemical data for CR.