EFFECTS OF ANTIOXIDANTS AND AGE ON OXUGEN STRESS BY VIGOROUS EXERCISE

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We studied antioxidative function in response to exercise by using animal and humans. In animal studies. we observed increased level of lipid peroxide in plasma and skeletal muscle after 90-min swimming exercise, and lower level of plasma vitamin C was observed rats one day after swimming. One bout of down-hill running in rats induced higher increase of plasma CPK activity with higher level of plasma lipid peroxide, and although plasma CPK activity returned to the resting level 48 hr after tunning, plasma antiocidant vitamines remained to the lower level compared with pre-exercise state. We evaluated DNA damage induced by one bout or repeated bouts of vigorous exercise in trained and untrained men. Spontaneous DNA damage in lymphocytes did not increase until 30 min after one bout of vigorous running in both trained and untrained men. The trained subjects had higher spontaneous DNA damage in lymphocytes and higher plasma CPK activity at the resting stale. The spontaneous DNA damage showed significant posotive correlation with plasma CPK activity, indicating that oxidative DNA damage in lymphocytes could be occured by extensive exercise, and might be closely associated with muscle damage. In addition, well-trained smimmers who had adequate antioxidative vitamins (vitamin C : 200-290mg/day, vitamin E : 7-12mg/day) maintained in the normal range of plasma vitamins (vitamin C : 1.1mg/dl, vitamin E : 11-12ug/ml). These studies suggest that adequate intke of antioxidative vitamins might be required for protecting oxidative damage induced by exercise.