Regulation of Gene Expression

(1)免疫学根据1989年与法国CNRS达成的协议，1994年首次开展了免疫学领域的联合研究计划。在1996年财政年度，研究集中在以下四个方面：a）T细胞分化、T细胞耐受和自身免疫性疾病发病机制的分子基础; B）B细胞发育过程中信号转导级联和基因表达的调节; c）通过抗原受体复合物进行信号转导的分子基础; d）免疫系统的发育和发病机制。利用小鼠转基因技术已经取得了一些令人瞩目的成果，为建立转基因新领域做出了重要贡献。(2)研究了哺乳动物Polycomb组基因产物的功能。使用mel-18和bmi-1缺陷小鼠进行的遗传分析显示，这些基因产物是建立前后轴所需的协同作用。B）通过细胞培养和转基因系统鉴定BETAB 1-晶状体蛋白组织特异性表达所需的增强子元件。(3)化合物Thiosrepton可激活Thiosrepton结合蛋白的转录活性。硫代瑞普顿衍生物也具有相同的生物活性，这与Deala侧链的长度密切相关。(4)生物传感器利用乙酰胆碱酯酶（AChE）建立了检测痕量有机磷农药的高灵敏度生物传感器。

(1) ImmunologyUpon the agreement with CNRS,France in 1989, a joint research program in the field of immunology was first undertaken in 1994. In this fiscal year 1996 the research was focused on the following 4 points : a) Molecular basis for T cell differentiation, T cell tolerance, and pathogenesis of autoimmune disease ; b) Regulation of signal transducing cascade and gene expression during B cell development ; c) Molecular basis underlying signal transduction via antigen receptor complexes ; and d) Development and pathogenesis of immune system. Several remarkable achievements were established by using mouse transgenic techniques and they greatly contributed the establishment of new region of transgenics.(2) Embryologya) Functions of mammalian Polycomb group gene products were investigated. Genetic analysis using mel-18 and bmi-1 deficient mice revealed that these gene products were synergically required for the establishment of anterior-posterior axis. b) Enhancer elements required for the tissue specific expression of BETAB1-crystallin were identified by using cell culture and transgenic system.(3) Molecvlar RecognitionThe chemical compound, Thiosrepton, activated transcriptional activity of Thiosrepton-binding protein. Derivatives of Thiosrepton also possessed the same biological activity which was strongly correlated with the length of Deala-side chain.(4) BiosensorThe highly sensitive biosensor to detect the trace of an insecticide containing organic phosphorus was developed by using Acetylcholine esterace (AChE).