Development of mental and physical biomarkers and assessment of its usefulness

为了开发普通人群的精神和身体生物标志物，我们测量和评估了精神和身体健康状况、外周血淋巴细胞自然杀伤（NK）细胞活性、血清总IgE水平、血液淋巴细胞染色体改变和氧化损伤（8OHdG）、尿液致突变活性、唾液皮质醇和IgA水平以及与生活方式密切相关的基因型（如ALDH2基因型）的有用性。在1995年1月阪神-淡路地震中遭受痛苦的成年男性被纳入研究。生活方式和创伤后应激障碍（PTSD）评分均与NK细胞活性相关。同样，血浆皮质醇水平与日本气象厅（JMA）的地震强度有关。一般健康问卷（GHQ）得分也与地震烈度有关。在日本成年男性工人中，唾液皮质醇水平与主观压力水平相关。在同一研究对象中，唾液IgA水平也与主观压力水平有关。这些观察结果表明，本研究中使用的神经学和免疫学生物标志物可用于评估健康受试者的健康状况，并可作为不良结果的预测指标。

In order to develop mental and physical biomarkers in a general population, we measured and evaluated usefulness of mental and physical health status, natural killer (NK) cell activity of peripheral blood lymphocytes, serum total IgE levels, chromosome alterations and oxidative damage (8OHdG) in blood lymphocytes, urinary mutagenic activity, salivary cortisol and IgA levels, and genotypes closely related to lifestyles such as the ALDH2 genotype.Male adults who suffered from Hanshin-Awaji Earthquake in Jan. 1995 were enrolled in the studies. Both lifestyles and post traumatic stress disorder (PTSD) score were related to NK cell activity. Likewise, plasma cortisol levels were linked with Japan Meteological Agency (JMA) Intensity of the earthquake. General Health Questionnaire (GHQ) score was also related with the intensity of the earthquake.In Japanese male adult workers, salivary cortisol levels were related with subjective stress levels. In the same subjects, salivary IgA levels were also associated with subjective stress levels.These observations indicate that the neurological and immunological biomarkers used in the present studies would be useful in the assessment of health status of healthy subjects and can be used as predictive indices for undesired outcomes.