Physiological roles of lymphatic endothelial cells on lymph formation and transport

活化巨噬细胞介导的内源性前列腺素与淋巴管平滑肌的一氧化氮依赖性舒张应用常规生物测定方法研究了细菌脂多糖（LPS）活化的巨噬细胞对有或无内皮的淋巴管机械活动的影响。巨噬细胞的上清液显著抑制由U46619预收缩的淋巴生物测定环的基础张力。巨噬细胞诱导的淋巴管的血管舒张作用显着减少了12小时的巨噬细胞与L-NAME，吲哚美辛，地塞米松，或放线菌酮预孵育。同时预孵育的L-NAME和吲哚美辛引起的巨噬细胞诱导的淋巴生物测定环的血管舒张的协同减少。这些发现表明，由细菌LPS激活的巨噬细胞通过共同释放一氧化氮和血管舒张性胰高血糖素产生淋巴管平滑肌的显著松弛。BAS ...更多信息 成纤维细胞生长因子介导的犬胸导管淋巴管内皮细胞的淋巴管生成：肝素的作用我们试图评估，与血管的血管生成相似，从狗胸导管分离的淋巴管内皮细胞（LEC）培养物具有响应碱性成纤维细胞生长因子（bFGF）诱导淋巴管生成的能力，然后检测肝素对bFGF的影响。介导的形态发生bFGF和/或肝素对LEC增殖和迁移的影响也分别通过改变亚融合细胞的数量和伤口迁移实验来评价。还通过相差显微镜和电子显微镜研究了试剂对LEC进入三维胶原凝胶的侵袭和管形成以及对胶原凝胶诱导的LEC管形成的影响。bFGF引起LEC的增殖和迁移的显著诱导，其诱导通过用范围为1至100 μ g/ml的肝素的额外处理而剂量依赖性地增强。碱性成纤维细胞生长因子产生的侵入和管形成的LEC成一个三维的胶原凝胶。bFGF还促进LEC在胶原凝胶层之间形成毛细血管样管。结果表明，从狗胸导管分离培养的LEC在bFGF的刺激下具有形成毛细淋巴管样淋巴管的能力，肝素可依赖性地促进bFGF介导的淋巴管新生。少

ACTIVATED MACROPHAGE-MEDIATED ENDOGENOUS PROSTAGLANDIN AND NITRIC OXIDE-DEPENDENT RELAXATION OF LYMPHATIC SMOOTH MUSCLESThe effects of macrophages activated by bacterial lipopolysaccharide (LPS) on the mechanical activity of lymph vessels with or without the endothelium were investigated using conventional bioassay preparations. The supematant of the macrophagtes suppressed significantly the basal tone of the lymphatic bioassay rings precontracted by U46619. The macrophage-induced vasodilation of the lymph vessels was significantly reduced by 12h preincubation of the macrophages with L-NAME,indomethacin, dexamethasone, or cycloheximide. Simultaneous preincubation of L-NAME and indomethacin caused a synergistic reduction of the macrophages-induced vasodilation of the lymphatic bioassay rings. These findings suggest that macrophages activated by bacterial LPS produce a marked relaxation of lymphatic smooth muscles through the co-release of nitric oxide and vasodilative prostaglandins.BAS … More ICPFIBROBLAST GROWTH FACTOR-MEDIATED LYMPHANGIOGENESIS OF LYMPHATIC ENDOTHELIAL CELLS ISOLATED FROM DOG THORACIC DUCTS : EFFECTS OF HEPARINWe have attempted to evaluate whether, similar to angiogenesis of blood vessels, cultures of lymphatic endothelial cells (LEC) isolated from dog thoracic ducts have an ability to induce lymphangiogenesis in response to basic fibroblast growth factor (bFGF) and then to examine effects of heparin on the bFGF-mediated morphogenesis. Effects of bFGF and/or heparin on proliferation and migration of the LEC were also evaluated by changing the number of the subconfluent cells and by wound migration assay, respectively. The effects of the agents on invasion and tube formation of the LEC into a three-dimensional collagen gel and on collagen gel-induced tube formation of the LEC were also investigated by a phase-contrast microscope and an electron microscope. The bFGF caused a significant induction of proliferation and migration of the LEC,the induction of which was augmented dose-dependently by an additional treatment with heparin ranging from 1 to 100 mug/ml. The bFGF produced invasion and tube formation of the LEC into a three-dimensional collagen gel. The bFGF also facilitated to form capillary-like tubes of the LEC between two layrs of colalgen gels. Heparin accelerated the both processes of the bFGF-mediated lymphangiogenesis of the LEC.These findings suggest that the cultured LEC isolated from dog thoracic ducts have an ability to form lymphatic capillary-like tubes in response to bFGF and that heparin accelerates does-dependently the process of the bFGF-mediated neovascularization of lymph vessels. Less